| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
HORMONES AND SIGNALING
1Cardiovascular and Metabolic Disease Research, Wyeth, Collegeville, Pennsylvania 19426; and 2Cardiovascular and Metabolic Disease Research and 3Inflammation Research, Wyeth, Cambridge, Massachusetts 02140
Submitted 16 January 2003 ; accepted in final form 28 August 2003
A well-established model of bowel inflammation is the HLA-B27 transgenic rat that exhibits a spontaneous disease phenotype resulting in chronic diarrhea caused by immune cell activation. Estrogens have previously been shown to modulate the immune system, and both estrogen receptors (ER
and ER
) are present in the intestine and cells of the immune system. Therefore, the ability of estrogen to ameliorate disease progression in the HLA-B27 transgenic rat was determined. HLA-B27 transgenic rats with chronic diarrhea were treated with 17-ethynyl-17-estradiol (EE) for 5 days. EE treatment dramatically improved stool scores after only 3 days. Histological scores of the degree of ulceration, inflammatory cell infiltration, fibrosis, and lesion depth of the colon were also improved by EE treatment. Because neutrophil infiltration into the colon is involved in the development and propagation of disease, myeloperoxidase (MPO) activity was measured. MPO levels were reduced by 80% by EE treatment. Cotreatment with the pure ER antagonist ICI-182780 (ICI) blocked the effects of EE on stool character, MPO activity, and histology scores, strongly suggesting that the activity of EE is mediated through ER. Mast cell proteases can promote neutrophil infiltration, and gene expression analysis demonstrated that mast cell protease 1, 3, and 4 mRNA were all decreased in colons from estrogen-treated rats. In addition, a direct effect of estrogen on bone marrow-derived mast cell activity was demonstrated, suggesting that ER-mediated inactivation of mast cells may contribute to the improvement in the clinical sign and histological scores in this model.
mast cells; estrogen receptor
This article has been cited by other articles:
|
|
 |
|
  M. Imaoka, M. Kato, S. Tago, M. Gotoh, H. Satoh, and S. Manabe Effects of Estradiol Treatment and/or Ovariectomy on Spontaneous Hemorrhagic Lesions in the Pancreatic Islets of Sprague-Dawley Rats Toxicol Pathol, February 1, 2009; 37(2): 218 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-P. Yu, Y.-C. Hsieh, T. Suzuki, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway J. Leukoc. Biol., September 1, 2007; 82(3): 774 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J Ma, E. A Guzman, A. DeGuzman, H K. Muller, A. M Walker, and L. B Owen Local cytokine levels associated with delayed-type hypersensitivity responses: modulation by gender, ovariectomy, and estrogen replacement J. Endocrinol., May 1, 2007; 193(2): 291 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-L. Lu, J.-C. Hsieh, M.-L. Tsaur, Y.-H. Huang, P. S. Wang, L.-L. Wu, P.-Y. Liu, F.-Y. Chang, and S.-D. Lee Estrogen rapidly modulates mustard oil-induced visceral hypersensitivity in conscious female rats: a role of CREB phosphorylation in spinal dorsal horn neurons Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G438 - G446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Winnard, N. Dmitrieva, and K. J. Berkley Cross-organ interactions between reproductive, gastrointestinal, and urinary tracts: modulation by estrous stage and involvement of the hypogastric nerve Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1592 - R1601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Yu, T. Shimizu, Y.-C. Hsieh, T. Suzuki, M. A. Choudhry, M. G. Schwacha, and I. H. Chaudry Tissue-specific expression of estrogen receptors and their role in the regulation of neutrophil infiltration in various organs following trauma-hemorrhage J. Leukoc. Biol., May 1, 2006; 79(5): 963 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Ph. Lesclous, F. Schramm, S. Gallina, B. Baroukh, D. Guez, and J. L. Saffar Histamine mediates osteoclastic resorption only during the acute phase of bone loss in ovariectomized rats Exp Physiol, May 1, 2006; 91(3): 561 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Janicki, G. L. Brower, J. D. Gardner, M. F. Forman, J. A. Stewart Jr., D. B. Murray, and A. L. Chancey Cardiac mast cell regulation of matrix metalloproteinase-related ventricular remodeling in chronic pressure or volume overload Cardiovasc Res, February 15, 2006; 69(3): 657 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Yu, M. A. Choudhry, T. Shimizu, Y.-C. Hsieh, M. G. Schwacha, S. Yang, and I. H. Chaudry Mechanism of the salutary effects of flutamide on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of estrogen receptor-{beta}-dependent HO-1 J. Leukoc. Biol., February 1, 2006; 79(2): 277 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Chancey, J. D. Gardner, D. B. Murray, G. L. Brower, and J. S. Janicki Modulation of cardiac mast cell-mediated extracellular matrix degradation by estrogen Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H316 - H321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Chadwick, S. Chippari, E. Matelan, L. Borges-Marcucci, A. M. Eckert, J. C. Keith Jr., L. M. Albert, Y. Leathurby, H. A. Harris, R. A. Bhat, et al. Identification of pathway-selective estrogen receptor ligands that inhibit NF-{kappa}B transcriptional activity PNAS, February 15, 2005; 102(7): 2543 - 2548. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
