HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 286/1/G37    most recent 00041.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Paxian, M. Articles by Clemens, M. G. Search for Related Content PubMed PubMed Citation Articles by Paxian, M. Articles by Clemens, M. G.

LIVER AND BILIARY TRACT

High-resolution visualization of oxygen distribution in the liver in vivo

¹Department of Biology, University of North Carolina, Charlotte 28223 and ²Department of Surgery, Carolinas Medical Center, Charlotte, North Carolina 28203

Submitted 24 January 2003 ; accepted in final form 20 March 2003

Microcirculatory failure after stress events results in mismatch in oxygen supply and demand. Determination of tissue oxygen distribution in vivo may help elucidate mechanisms of injury, but present methods have limited resolution. Male Sprague-Dawley rats were anesthetized, prepared for intravital microscopy, and received intravenously the oxygen-sensitive fluorescent dye Tris(1,10-phenanthroline)ruthenium(II) chloride hydrate []. An impaired hepatic oxygen distribution was induced by either phenylephrine or hemorrhage. Intensity of fluorescence was compared with NADH autofluorescence indicating changes in the mitochondrial redox potential. Ethanol was injected to affect the NADH-to-NAD⁺ ratio without altering the PO₂. Infusion of resulted in a heterogeneous fluorescence under baseline conditions reflecting the physiological acinar PO₂ distribution. A decrease in oxygen supply due to phenylephrine or hemorrhage was paralleled by an increase in and NADH fluorescence reflecting an impaired mitochondrial redox state. Ethanol did not alter fluorescence but increased NADH fluorescence indicating independence of PO₂ and redox state imaging. Intravenous administration of for intravital videomicroscopy represents a new method to visualize the hepatic tissue PO₂. Combined with NADH autofluorescence, it provides additional information regarding the tissue redox state.

aminotransferase; fluorescence microscopy; in vivo imaging; hemorrage; ethanol

This article has been cited by other articles:

				B. Vollmar and M. D. Menger The Hepatic Microcirculation: Mechanistic Contributions and Therapeutic Targets in Liver Injury and Repair Physiol Rev, October 1, 2009; 89(4): 1269 - 1339. [Abstract] [Full Text] [PDF]

				L. Wu, N. Gokden, and P. R. Mayeux Evidence for the Role of Reactive Nitrogen Species in Polymicrobial Sepsis-Induced Renal Peritubular Capillary Dysfunction and Tubular Injury J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1807 - 1815. [Abstract] [Full Text] [PDF]

				L. Wu, M. M. Tiwari, K. J. Messer, J. H. Holthoff, N. Gokden, R. W. Brock, and P. R. Mayeux Peritubular capillary dysfunction and renal tubular epithelial cell stress following lipopolysaccharide administration in mice Am J Physiol Renal Physiol, January 1, 2007; 292(1): F261 - F268. [Abstract] [Full Text] [PDF]