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Acidic duodenal pH alters gene expression in the cystic fibrosis mouse pancreas

¹Department of Anatomy and Cell Biology, School of Medicine, University of Kansas, Kansas City, Kansas 66160; and ²Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan

Submitted 22 January 2004 ; accepted in final form 26 March 2004

The duodenum is abnormally acidic in cystic fibrosis (CF) due to decreased bicarbonate ion secretion that is dependent on the CF gene product CFTR. In the CFTR null mouse, the acidic duodenum results in increased signaling from the intestine to the exocrine pancreas in an attempt to stimulate pancreatic bicarbonate ion secretion. Excess stimulation is proposed to add to the stress/inflammation of the pancreas in CF. DNA microarray analysis of the CF mouse revealed altered pancreatic gene expression characteristic of stress/inflammation. When the duodenal pH was corrected genetically (crossing CFTR null with gastrin null mice) or pharmacologically (use of the proton pump inhibitor omeprazole), expression levels of genes measured by quantitative RT-PCR were significantly normalized. It is concluded that the acidic duodenal pH in CF contributes to the stress on the exocrine pancreas and that normalizing duodenal pH reduces this stress.

inflammation; leucine-rich ₂-glycoprotein; Muclin; pancreatitis-associated protein; regenerating islet-derived genes

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