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Neuronal activity and CRF receptor gene transcription in the brains of rats with colitis

¹Groupe d'Etude du Stress et des Interactions Neuro-Digestives (EA 3744), and ²Département d'Hépato-Gastroenterologie, Hôpital Albert Michallon, Centre Hospitalier Universitaire, Cedex 09, France

Submitted 29 March 2004 ; accepted in final form 1 June 2004

We aimed to characterize neuronal and corticotropin-releasing factor (CRF) pathways at the acute phase of a model of colitis in rats. Male rats received an intracolonic injection of either vehicle (controls) or trinitrobenzenesulfonic acid (TNBS) and were killed 1, 2, 3, 4, 6, 12, or 24 h later. Coronal frozen sections of the brain were cut and mRNAs encoding the rat c-fos, CRF₁ receptor, and CRF_2, receptors were assayed by in situ hybridization histochemistry. Localization of these transcripts within CRF-immunoreactive (CRF-ir) neurons of the paraventricular nucleus (PVN) of the hypothalamus was also determined. Intracolonic TNBS induced c-fos mRNA expression in brain nuclei involved in the autonomic, behavioral, and neuroendocrine response to a stimulus (PVN, amygdala, locus coeruleus, parabrachial nucleus, nucleus of the solitary tract) and in circumventricular organs (lamina terminalis, subfornical organ, area postrema). CRF pathways, particularly in the PVN, were activated in this model as represented by a robust signal of c-fos and CRF₁ receptor transcripts in the PVN and numerous CRF-ir neurons expressed c-fos or CRF₁ receptor transcripts in the PVN of TNBS-treated animals. No expression of CRF₂ receptor transcripts was observed in the PVN, either in basal conditions or after TNBS. These neuroanatomical data argue for an involvement of CRF pathways, through CRF₁ receptor, within the PVN in TNBS-induced colitis.

c-fos; colon; hypothalamus; inflammation; in situ hybridization; stress

This article has been cited by other articles:

				C. Porcher, A. Juhem, A. Peinnequin, V. Sinniger, and B. Bonaz Expression and effects of metabotropic CRF1 and CRF2 receptors in rat small intestine Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1091 - G1103. [Abstract] [Full Text] [PDF]