HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/2/G354    most recent 00356.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Lay, J. M. Articles by Samuelson, L. C. Search for Related Content PubMed PubMed Citation Articles by Lay, J. M. Articles by Samuelson, L. C.

HORMONES AND SIGNALING

Enteroendocrine cell expression of a cholecystokinin gene construct in transgenic mice and cultured cells

¹Department of Molecular and Integrative Physiology and ²Graduate Program in Cellular and Molecular Biology, The University of Michigan, Ann Arbor, Michigan

Submitted 6 August 2004 ; accepted in final form 8 October 2004

CCK is predominantly expressed in subsets of endocrine cells in the intestine and neurons in the brain. We evaluated the expression of a CCK gene construct in transgenic mice and cultured cells to identify a genomic region that directs correct tissue- and cell-specific expression in enteroendocrine cells. The CCKL1 transgene contained 6.4 kb of mouse Cck fused to lacZ. Expression was evaluated in three transgenic lines (J11, J12, J14) by measurement of -galactosidase in tissue homogenates and frozen sections. Correct tissue-specific expression was observed, with -galactosidase activity detected in intestine and brain. However, there were differences seen in cell-specific expression in the intestine. Line J14 exhibited expression in CCK-endocrine cells, with expressing cells arising at the normal time during fetal development. However, transgene expression in line J12 intestine was limited to neurons of the enteric nervous system, which reflect an early fetal expression pattern for CCK. Analysis of an additional 15 transgenic founder mice demonstrated intestinal expression in 40% of transgenics, with expressing mice following either an endocrine cell pattern or a neuronal pattern in approximately equal numbers. CCKL1 transfection analysis in cultured cells also demonstrated enteroendocrine cell expression, with 100-fold enhanced activity in the enteroendocrine cell line STC-1 compared with nonendocrine cell lines. The results suggest that the minimal cis-regulatory DNA elements necessary for appropriate CCK expression in enteroendocrine cells reside within the 6.4-kb mouse genomic fragment.

intestine; endocrine cells; enteric nervous system; gene expression

This article has been cited by other articles:

				M. C. Chen, S. V. Wu, J. R. Reeve Jr., and E. Rozengurt Bitter stimuli induce Ca2+ signaling and CCK release in enteroendocrine STC-1 cells: role of L-type voltage-sensitive Ca2+ channels Am J Physiol Cell Physiol, October 1, 2006; 291(4): C726 - C739. [Abstract] [Full Text] [PDF]