AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 289: G42-G53, 2005. First published March 3, 2005; doi:10.1152/ajpgi.00500.2004
0193-1857/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
289/1/G42    most recent
00500.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bradesi, S.
Right arrow Articles by Mayer, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bradesi, S.
Right arrow Articles by Mayer, E. A.

NEUROREGULATION AND MOTILITY

Repeated exposure to water avoidance stress in rats: a new model for sustained visceral hyperalgesia

Sylvie Bradesi,1,4 Ines Schwetz,1 Helena S. Ennes,1,4 Christophe M. R. Lamy,1,4 Gordon Ohning,1,4 Michael Fanselow,3 Charalabos Pothoulakis,5 James A. McRoberts,1,4 and Emeran A. Mayer1,2,3,4

1Departments of Medicine, 2Physiology, 3Psychiatry and Biobehavioral Sciences and Brain Research Institute, Center for Neurovisceral Sciences and Women’s Health and 4Center for Ulcer Research and Education: Digestive Diseases Research Center, David Geffen School of Medicine at University of California, Greater Los Angeles Veterans Affairs Healthcare System, Los Angeles, California; and 5Gastrointestinal Neuropeptide Center, Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts

Submitted 8 November 2004 ; accepted in final form 25 February 2005

Chronic stress plays an important role in the development and exacerbation of symptoms in functional gastrointestinal disorders. To better understand the mechanisms underlying this relationship, we aimed to characterize changes in visceral and somatic nociception, colonic motility, anxiety-related behavior, and mucosal immune activation in rats exposed to 10 days of chronic psychological stress. Male Wistar rats were submitted daily to either 1-h water avoidance (WA) stress or sham WA for 10 consecutive days. The visceromotor response to colorectal distension, thermal somatic nociception, and behavioral responses to an open field test were measured at baseline and after chronic WA. Fecal pellets were counted after each WA stress or sham WA session as a measure of stress-induced colonic motility. Colonic samples were collected from both groups and evaluated for structural changes and neutrophil infiltration, mast cell number by immunohistochemistry, and cytokine expression by quantitative RT-PCR. Rats exposed to chronic WA (but not sham stress) developed persistent visceral hyperalgesia, whereas only transient changes in somatic nociception were observed. Chronically stressed rats also exhibited anxiety-like behaviors, enhanced fecal pellet excretion, and small but significant increases in the mast cell numbers and the expression of IL-1{beta} and IFN-{gamma}. Visceral hyperalgesia following chronic stress persisted for at least a month. Chronic psychological stress in rats results in a robust and long-lasting alteration of visceral, but not somatic nociception. Visceral hyperalgesia is associated with other behavioral manifestations of stress sensitization but was only associated with minor colonic immune activation arguing against a primary role of mucosal immune activation in the maintenance of this phenomenon.

chronic psychological stress; visceral nociception


Address for reprint requests and other correspondence: E. A. Mayer, Center for Neurovisceral Sciences and Women’s Health, Greater Los Angeles Veterans Affairs Healthcare System, Center for Ulcer Research and Education, Bldg. 115/Rm. 223, 11301 Wilshire Blvd., Los Angeles, CA 90073 (E-mail: emayer{at}ucla.edu)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Larauche, S. Bradesi, M. Million, P. McLean, Y. Tache, E. A. Mayer, and J. A. McRoberts
Corticotropin-releasing factor type 1 receptors mediate the visceral hyperalgesia induced by repeated psychological stress in rats
Am J Physiol Gastrointest Liver Physiol, April 1, 2008; 294(4): G1033 - G1040.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
E A Mayer, S Bradesi, L Chang, B M R Spiegel, J A Bueller, and B D Naliboff
Functional GI disorders: from animal models to drug development
Gut, March 1, 2008; 57(3): 384 - 404.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. Boudry, C. I. Cheeseman, and M. H. Perdue
Psychological stress impairs Na+-dependent glucose absorption and increases GLUT2 expression in the rat jejunal brush-border membrane
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R862 - R867.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.