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LIVER AND BILIARY TRACT

NF-B activation in Kupffer cells after partial hepatectomy

¹Department of Medicine, University of North Carolina, Chapel Hill, North Carolina; and ²Department of Medicine, Columbia University, New York, New York

Submitted 29 November 2004 ; accepted in final form 4 April 2005

The transcription factor nuclear factor-B (NF-B) is activated during liver regeneration after partial hepatectomy. However, the physiological role and cellular localization of NF-B activation are unresolved. In this study, we used an adenoviral vector expressing a mutated form of IB to inhibit NF-B activity during liver regeneration. After partial hepatectomy in mice, introduction of Ad5IB, but not a control virus (Ad5GFP), resulted in increased liver injury and decreased hepatocyte proliferation. Hepatocyte apoptosis was not observed. To investigate the kinetics and cellular localization of NF-B-induced transcription during liver regeneration, we generated a transgenic mouse expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-B cis elements (cis-NF-B-EGFP). During liver regeneration, EGFP expression was detected within 12 h and was primarily located in Kupffer cells. Our data demonstrate that activation of NF-B initially occurs in Kupffer cells after partial hepatectomy in mice.

liver regeneration; nuclear factor-; B; Kupffer cell

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