HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Huang, B. Q. Articles by LaRusso, N. F. Search for Related Content PubMed PubMed Citation Articles by Huang, B. Q. Articles by LaRusso, N. F.

LIVER AND BILIARY TRACT

Isolation and characterization of cholangiocyte primary cilia

Center for Basic Research in Digestive Disease, Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, Minnesota

Submitted 8 February 2006 ; accepted in final form 4 April 2006

Primary cilia are distinct organelles expressed by many vertebrate cells, including cholangiocytes; however, their functions remain obscure. To begin to explore the physiological role of these organelles in the liver, we described the morphology and structure of cholangiocyte cilia and developed new approaches for their isolation. Primary cilia were present only in bile ducts and were not observed in hepatocytes or in hepatic arterial or portal venous endothelial cells. Each cholangiocyte possesses a single cilium that extends from the apical membrane into the bile duct lumen. In addition, the length of the cilia was proportional to the bile duct diameter. We reproducibly isolated enriched fractions of cilia from normal rat and mouse cholangiocytes by two different approaches as assessed by scanning electron, transmission electron, and confocal microscopy. The purity of isolated ciliary fractions was further analyzed by Western blot analysis using acetylated tubulin as a ciliary marker and P2Y₂ as a nonciliary cell membrane marker. These novel techniques produced enriched ciliary fractions of sufficient purity and quantity for light and electron microscopy and for biochemical analyses. They will permit further assessment of the role of primary cilia in normal and pathological conditions.

cilia isolation; fibrocystin; polycystins

This article has been cited by other articles:

				A. I. Masyuk, S. A. Gradilone, J. M. Banales, B. Q. Huang, T. V. Masyuk, S.-O. Lee, P. L. Splinter, A. J. Stroope, and N. F. LaRusso Cholangiocyte primary cilia are chemosensory organelles that detect biliary nucleotides via P2Y12 purinergic receptors Am J Physiol Gastrointest Liver Physiol, October 1, 2008; 295(4): G725 - G734. [Abstract] [Full Text] [PDF]

				K. Woo, A. K. Dutta, V. Patel, C. Kresge, and A. P. Feranchak Fluid flow induces mechanosensitive ATP release, calcium signalling and Cl- transport in biliary epithelial cells through a PKC{zeta}-dependent pathway J. Physiol., June 1, 2008; 586(11): 2779 - 2798. [Abstract] [Full Text] [PDF]

				A. de Souza Otero and G. Szabo Omission of citing prior work Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1813 - G1813. [Full Text] [PDF]

				L.-W. Tam, N. F. Wilson, and P. A. Lefebvre A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas J. Cell Biol., March 12, 2007; 176(6): 819 - 829. [Abstract] [Full Text] [PDF]