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Am J Physiol Gastrointest Liver Physiol 291: G735-G743, 2006; doi:10.1152/ajpgi.00388.2005
0193-1857/06 $8.00
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INFLAMMATION/IMMUNITY/MEDIATORS

Increased expression of midkine in the rat colon during healing of experimental colitis

Takafumi Yuki, Shunji Ishihara, M. A. K. Rumi, Cesar F. Ortega-Cava, Yasunori Kadowaki, Hideaki Kazumori, Norihisa Ishimura, Yuji Amano, Nobuyuki Moriyama, and Yoshikazu Kinoshita

Department of Gastroenterology and Hepatology, Shimane University School of Medicine, Shimane, Japan

Submitted 22 August 2005 ; accepted in final form 29 March 2006

Midkine (MK) is a unique growth and differentiation factor that modulates the proliferation and migration of various cells; however, little is known regarding its relationship to intestinal diseases. The aim of this study was to investigate MK expression and its role in dextran sulfate sodium (DSS)-induced colitis in rats. The expressions of MK, receptor-like protein-tyrosine phosphatase (RPTP)-beta, and proinflammatory cytokines were examined in rat colonic tissues after the development of DSS-induced colitis using Northern blotting, immunohistochemistry, and laser-capture microdissection (LCM) coupled with RT-PCR. The effects of MK on the migration of intestinal epithelial cells (IEC-6) were also evaluated in vitro using an intestinal wound repair model. MK expression was significantly increased in damaged colonic mucosa, mainly from day 3 to day 5 after the end of DSS administration, with abundant MK immunoreactive signals detected in submucosal fibroblasts. Expressions of proinflammatory cytokines were most strongly induced on day 1, which preceded the augmentation of MK expression. Results of LCM coupled with RT-PCR clearly indicated RPTP-beta expression in colonic epithelial cells. The migration assay showed that wound repair in the MK-treated groups was accelerated dose dependently. The present results showed for the first time that intestinal inflammation upregulates the MK-RPTP-beta system, which may stimulate mucosal regeneration during the process of healing of colitis. Additional investigations regarding the role of MK may contribute to the development of new options for the treatment of inflammatory bowel diseases.

receptor-like protein-tyrosine phosphatase-beta; proinflammatory cytokines; inflammatory bowel diseases; dextran sulfate sodium-induced colitis


Address for reprint requests and other correspondence: S. Ishihara, Dept. of Gastroenterology and Hepatology, Shimane Medical Univ. School of Medicine, 89-1, Enya-cho, Izumo, Shimane 693-0021, Japan (e-mail: si360405{at}med.shimane-u.ac.jp)






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