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Am J Physiol Gastrointest Liver Physiol 292: G899-G904, 2007. First published December 7, 2006; doi:10.1152/ajpgi.00398.2006
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MUCOSAL BIOLOGY

Isoflurane-induced acidosis depresses basal and PGE₂-stimulated duodenal bicarbonate secretion in mice

Division of Physiology, Department of Neuroscience, Uppsala University, Uppsala, Sweden

Submitted 26 August 2006 ; accepted in final form 30 November 2006

When running in vivo experiments, it is imperative to keep arterial blood pressure and acid-base parameters within the normal physiological range. The aim of this investigation was to explore the consequences of anesthesia-induced acidosis on basal and PGE₂-stimulated duodenal bicarbonate secretion. Mice (strain C57bl/6J) were kept anesthetized by a spontaneous inhalation of isoflurane. Mean arterial blood pressure (MAP), arterial acid-base balance, and duodenal mucosal bicarbonate secretion (DMBS) were studied. Two intra-arterial fluid support strategies were used: a standard Ringer solution and an isotonic Na₂CO₃ solution. Duodenal single perfusion was used, and DMBS was assessed by back titration of the effluent. PGE₂ was used to stimulate DMBS. In Ringer solution-infused mice, isoflurane-induced acidosis became worse with time. The blood pH was 7.15–7.21 and the base excess was about –8 mM at the end of experiments. The continuous infusion of Na₂CO₃ solution completely compensated for the acidosis. The blood pH was 7.36–7.37 and base excess was about 1 mM at the end of the experiment. Basal and PGE₂-stimulated DMBS were markedly greater in animals treated with Na₂CO₃ solution than in those treated with Ringer solution. MAP was slightly higher after Na₂CO₃ solution infusion than after Ringer solution infusion. We concluded that isoflurane-induced acidosis markedly depresses basal and PGE₂-stimulated DMBS as well as the responsiveness to PGE₂, effects prevented by a continuous infusion of Na₂CO₃. When performing in vivo experiments in isoflurane-anesthetized mice, it is recommended to supplement with a Na₂CO₃ infusion to maintain a normal acid-base balance.

anesthesia; murine physiology; in vivo; duodenal mucosal protection

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