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MUCOSAL BIOLOGY
Departments of 1Biochemistry, 2Diabetes and Endocrinology, and 5Morphology, Faculty of Medicine, and 3School of Medical Technology and Health, Faculty of Health and Medical Care, Saitama Medical University, Saitama, Japan; 4Burnham Institute for Medical Research, La Jolla, California; 6Toxicology Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco, Kanagawa, Japan; and 7Department of Medicine, Washington University School of Medicine, St. Louis, Missouri
Submitted 24 July 2006 ; accepted in final form 3 October 2006
Intestinal alkaline phosphatase (IAP) is involved in the process of fat absorption, a conclusion confirmed by an altered lipid transport and a faster body weight gain from 10 to 30 wk in both male and female mice with a homozygous null mutation of the IAP coding gene (Akp3–/– mice). This study was aimed to delineate morphologically and quantitatively the accelerated lipid absorption in male Akp3–/– mice. Feeding a corn oil bolus produced an earlier peak of triacylglycerol in serum (2 vs. 4 h for Akp3–/– and wild-type mice, respectively) and an approximately twofold increase in serum triacylglycerol concentration in Akp3–/– mice injected with a lipolysis inhibitor, Triton WR-1339. A corn oil load induced the threefold enlargement of the Golgi vacuoles in male wild-type mice but not in Akp3–/– mice, indicating that absorbed lipids rarely reached the Golgi complex and that the transcytosis of lipid droplets does not follow the normal pathway in male Akp3–/– mice. Force feeding an exaggerated fat intake by a 30% fat chow for 10 wk induced obesity in both male Akp3–/– and wild-type mice, and therefore no phenotypic difference was observed between the two. On the other hand, the forced high-fat chow induced an 18% greater body weight gain, hepatic steatosis, and visceral fat accumulation in female Akp3–/– mice but not in female wild-type controls. These results provide further evidence that IAP is involved in the regulation of the lipid absorption process and that its absence leads to progressive metabolic abnormalities in certain fat-forced conditions.
fat absorption; metabolic abnormality; obesity; small intestine
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