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MUCOSAL BIOLOGY

Iron absorption by Belgrade rat pups during lactation

¹Department of Genetics and Complex Diseases and ²Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts

Submitted 10 April 2007 ; accepted in final form 18 July 2007

Divalent metal transporter-1 (DMT1) mediates dietary nonheme iron absorption. Belgrade (b) rats have defective iron metabolism due to a mutation in the DMT1 gene. To examine the role of DMT1 in neonatal iron assimilation, b/b and b/+ pups were cross-fostered to F344 Fischer dams injected with ⁵⁹FeCl₃ twice weekly during lactation. Tissue distribution of the radioisotope in the pups was determined at weaning (day 21). The b/b pups had blood ⁵⁹Fe levels significantly lower than b/+ controls but significantly higher ⁵⁹Fe tissue levels in heart, bone marrow, skeletal muscle, kidney, liver, spleen, stomach, and intestines. To study the pharmacokinetics of nonheme iron absorption at the time of weaning, ⁵⁹FeCl₃ was administered to 21-day-old b/b and b/+ rats by intragastric gavage. Blood ⁵⁹Fe levels measured 5 min to 4 h postgavage were significantly lower in b/b rats, consistent with impaired DMT1 function in intestinal iron absorption. Tissue ⁵⁹Fe levels were also lower in b/b rats postgavage. Combined, these data suggest that DMT1 function is not essential for iron assimilation from milk during early development in the rat.

iron transport; lactation; DMT1; Belgrade rat

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