AJP - GI AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 296: G455-G460, 2009. First published December 24, 2008; doi:10.1152/ajpgi.90381.2008
0193-1857/09 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
296/3/G455    most recent
90381.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Whitehead, R. H.
Right arrow Articles by Robinson, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Whitehead, R. H.
Right arrow Articles by Robinson, P. S.

REVIEWS

Establishment of conditionally immortalized epithelial cell lines from the intestinal tissue of adult normal and transgenic mice

Robert H. Whitehead and Pamela S. Robinson

Novel Cell Line Core Facility, Vanderbilt Digestive Disease Research Center, Division of Gastroenterology, Vanderbilt University, Nashville, Tennessee

Submitted 17 June 2008 ; accepted in final form 18 December 2008

ABSTRACT

It has proved to be impossible to culture epithelial cells from the gastrointestinal tract of adult animals. Researchers have had to use either cell lines derived from newborn rat small intestine or colon carcinoma cell lines that have retained some of the properties of the gastrointestinal mucosa. We have described a method for establishing conditionally immortalized cell lines from the stomach, small intestine, colon, pancreas, and liver from tissue obtained from a transgenic mouse strain carrying a temperature-sensitive mutant of the SV40 large T gene (the "Immortomouse"). This immortalizing gene has proved to be useful for establishing cell lines from a number of transgenic mice following crossbreeding of the Immortomouse with the transgenic mouse of interest. These cell lines are being used in numerous studies. In this review we describe the methods for developing such lines and list the range of cell lines that have been developed from colon, small intestine, stomach, liver, and pancreas of a number of transgenic mice.

epithelium; intestine; mouse; tissue culture


Address for reprint requests and other correspondence: R. H. Whitehead, MRB IV, Rm. 1055, Vanderbilt Univ., Nashville, TN 37232 (e-mail: robert.whitehead{at}vanderbilt.edu) or P. S. Robinson, MRB IV, Rm. 1055, Vanderbilt Univ., Nashville, TN 37232 (e-mail: pamela.s.robinson{at}vanderbilt.edu)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. A. Blaine, K. C. Ray, K. M. Branch, P. S. Robinson, R. H. Whitehead, and A. L. Means
Epidermal growth factor receptor regulates pancreatic fibrosis
Am J Physiol Gastrointest Liver Physiol, September 1, 2009; 297(3): G434 - G441.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2009 by the American Physiological Society.