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NEUROREGULATION AND MOTILITY

Lysophosphatidyl choline modulates mechanosensitive L-type Ca²⁺ current in circular smooth muscle cells from human jejunum

¹Enteric Neuroscience Program, ²Miles and Shirley Fiterman Center for Digestive Diseases, and ³Gastroenterologic and General Surgery, Mayo Clinic, Rochester, Minnesota

Submitted 23 October 2008 ; accepted in final form 23 January 2009

The L-type Ca²⁺ channel expressed in gastrointestinal smooth muscle is mechanosensitive. Direct membrane stretch and shear stress result in increased Ca²⁺ entry into the cell. The mechanism for mechanosensitivity is not known, and mechanosensitivity is not dependent on an intact cytoskeleton. The aim of this study was to determine whether L-type Ca²⁺ channel mechanosensitivity is dependent on tension in the lipid bilayer in human jejunal circular layer myocytes. Whole cell currents were recorded in the amphotericin-perforated-patch configuration, and lysophosphatidyl choline (LPC), lysophosphatidic acid (LPA), and choline were used to alter differentially the tension in the lipid bilayer. Shear stress (perfusion at 10 ml/min) was used to mechanostimulate L-type Ca²⁺ channels. The increase in L-type Ca²⁺ current induced by shear stress was greater in the presence of LPC (large head-to-tail proportions), but not LPA or choline, than in the control perfusion. The increased peak Ca²⁺ current also did not return to baseline levels as in control conditions. Furthermore, steady-state inactivation kinetics were altered in the presence of LPC, leading to a change in window current. These findings suggest that changes in tension in the plasmalemmal membrane can be transmitted to the mechanosensitive L-type Ca²⁺ channel, leading to altered activity and Ca²⁺ entry in the human jejunal circular layer myocyte.

calcium channel; gastrointestinal; lipid bilayer tension; shear stress; lysophosphatidic acid