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Fig. 6. Disruption of subapical F-actin is unaffected by 8-Br-cAMP or Epac. A and D: pancreatic acini were stimulated with either carbachol or caerulein with or without 8-Br-cAMP (100 µM) or the Epac agonist 8-pCPT-2'-O-Me-cAMP (100 µM). Cells were fixed and stained with rhodamine-phalloidin (red) for F-actin and TOPRO-3 (blue) for nuclei. Representative images from 3 independent experiments are shown. B and E: apical intensities were measured for each sample and expressed relative to unstimulated cells. C and F: ratio of fluorescence intensities of the apical to basolateral regions were determined for each condition. Unstim., unstimulated; Carb, carbachol. Seven cells were analyzed per condition and expressed as means ± SE. *,#,P < 0.05 for either carbachol (1 mM) alone, with 8-Br-cAMP, or with 8-pCPT-2'-O-Me-cAMP compared with unstimulated, respectively.

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