Corrigendum

For this study, animals were anesthetized by an intraperitoneal injection of saturated sodium pentobarbital solution (50 mg/ml). Following attainment of deep anesthesia, the abdomen of the animal was opened and the liver exposed.

In the Articles in PresS version of this manuscript, Materials and Methods section, we have erroneously reported using the method of cervical dislocation, which is not consistent with the recommendations of the AMVA as a method of euthanasia in rats weighing greater than 200 g. Furthermore, even if accepted, the procedure is not conducive to proper isolation of the hepatocytes by collagenase circulation as it prevents an effective cannulation of the portal vein due to intra-vein blood stasis and coagulation. For this reason, a deep anesthesia (assessed by the lost of corneal reflex) was induced by i.p. injection of sodium pentobarbital as indicated above. Following attainment of deep anesthesia, the abdomen of the animal was opened and the liver explanted (for studies on whole tissue cation content), or the portal vein cannulated for proper in situ liver perfusion and hepatocyte isolation by collagenase digestion. In either case, euthanasia of the animals was ultimately achieved by exanguination.