AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol (November 6, 2003). doi:10.1152/ajpgi.00020.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/3/G395    most recent
00020.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reeve, Jr., J. R.
Right arrow Articles by Coskun, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reeve, Jr., J. R.
Right arrow Articles by Coskun, T.
Submitted on January 15, 2003
Accepted on September 17, 2003

Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

Joseph R. Reeve, Jr.1*, S. Vincent Wu1, David A. Keire1, Kym Faull2, Peter Chew1, Travis E. Solomon1, Gary M. Green3, and Tamer Coskun1

1 CURE: Digestive Diseases Research Center, VA GLAHS, Los Angeles, CA, USA; Digestive Diseases Division, UCLA School of Medicine, Los Angeles, CA, USA
2 Pasarow Mass Spectrometry Laboratory, Departments of Psychiatry & Biobehavioral Sciences and the Neuropsychiatric Institute, and Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA
3 Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

* To whom correspondence should be addressed. E-mail: jreeve{at}ucla.edu.

In this work we 1) synthesize rat CCK-58, 2) determine the amounts and forms of rat cholecystokinin in whole blood after stimulation of its release by casein, 3) determine the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCK-A and CCK-B receptors transfected into CHO cells, and 4) examine the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of cholecystokinin in rat blood. Synthetic rat CCK-58 was less potent that CCK-8 for displacing label from CCK-A and CCK-B receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58, but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the C-terminus of rat CCK-58 influence the expression of cholecystokinin biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of cholecystokinin activity.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. V. Wu, K. G. Harikumar, R. J. Burgess, J. R. Reeve Jr., and L. J. Miller
Effects of cholecystokinin-58 on type 1 cholecystokinin receptor function and regulation
Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G641 - G647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. N. Sullivan, S. J. Raboin, S. Gulley, N. T. Sinzobahamvya, G. M. Green, J. R. Reeve Jr., and A. I. Sayegh
Endogenous cholecystokinin reduces food intake and increases Fos-like immunoreactivity in the dorsal vagal complex but not in the myenteric plexus by CCK1 receptor in the adult rat
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1071 - R1080.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Yamamoto, J. R. Reeve Jr., D. A. Keire, and G. M. Green
Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8
Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G866 - G879.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1979 by the American Physiological Society.