| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Surgery, UMDNJ - New Jersey Medical School, Newark, NJ, USA
* To whom correspondence should be addressed. E-mail: edeitch{at}umdnj.edu.
Having documented that proestrus female rats are more resistant to shock-induced acute gut and hence lung injury than male rats, we tested the hypothesis that the female gut is more resistant to injury and produces less of an inflammatory response than the male gut when exposed to conditions associated with shock states (hypoxia and acidosis) utilizing the ex-vivo Ussing chamber system. Ileal mucosal membranes harvested from normal male and female rats mounted in Ussing chamber systems were exposed to normoxia or 40 min of hypoxia at a normal pH (pH 7.3) or acidosis (pH6.8). Cytokine and nitric oxide levels in the serosal compartment of the Ussing chamber were measured at the end of the 3 hr hour experimental period to assess the immuno-inflammatory response, while FITC-dextran (MW 4,300) was employed to assess barrier function. Histo-morphological changes were used to quantitate gut mucosal injury. Hypoxia, acidosis or hypoxia plus acidosis was associated with a significant increase in pro-inflammatory cytokine production (IL-6, TNF, MIP-2) by the male as compared with the female intestinal segments. In contrast, the female gut manifested a higher anti-inflammatory response (nitric oxide and IL-10) and improved intestinal barrier function as well as less evidence of mucosal injury than the male intestinal segments. Administration of estradiol or the testosterone receptor antagonist, flutamide, to male rats abrogated the increase in gut injury and the increased IL-6 and MIP-2 response observed after hypoxia plus acidosis. These results suggest that gender differences in the ex vivo intestinal response to stresses, such as hypoxia and acidosis, exist and that the administration of estradiol or blockade of the testosterone receptor to male rats mitigates these gender-differences.
This article has been cited by other articles:
|
|
 |
|
  S. Akhtar, X. Li, I. H. Chaudry, and M. A. Choudhry Neutrophil chemokines and their role in IL-18-mediated increase in neutrophil O2- production and intestinal edema following alcohol intoxication and burn injury Am J Physiol Gastrointest Liver Physiol, August 1, 2009; 297(2): G340 - G347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Braniste, M. Leveque, C. Buisson-Brenac, L. Bueno, J. Fioramonti, and E. Houdeau Oestradiol decreases colonic permeability through oestrogen receptor \#946;-mediated up-regulation of occludin and junctional adhesion molecule-A in epithelial cells J. Physiol., July 1, 2009; 587(13): 3317 - 3328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, S. Yang, S. Hu, M. A. Choudhry, K. I. Bland, and I. H. Chaudry Estrogen prevents intestinal inflammation after trauma-hemorrhage via downregulation of angiotensin II and angiotensin II subtype I receptor Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G1131 - G1137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. I. Spratt, R. S. Kramer, J. R. Morton, F. L. Lucas, K. Becker, and C. Longcope Characterization of a prospective human model for study of the reproductive hormone responses to major illness Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E63 - E69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-T. Hsu, W.-H. Kan, C.-H. Hsieh, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry Mechanism of estrogen-mediated intestinal protection following trauma-hemorrhage: p38 MAPK-dependent upregulation of HO-1 Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1825 - R1831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-P. Yu, Y.-C. Hsieh, T. Suzuki, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway J. Leukoc. Biol., September 1, 2007; 82(3): 774 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Spratt, J. R. Morton, R. S. Kramer, S. W. Mayo, C. Longcope, and C. P. H. Vary Increases in serum estrogen levels during major illness are caused by increased peripheral aromatization Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E631 - E638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Yu, T. Shimizu, Y.-C. Hsieh, T. Suzuki, M. A. Choudhry, M. G. Schwacha, and I. H. Chaudry Tissue-specific expression of estrogen receptors and their role in the regulation of neutrophil infiltration in various organs following trauma-hemorrhage J. Leukoc. Biol., May 1, 2006; 79(5): 963 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Blackwell, S. M. Moscovis, A. E. Gordon, O. M. Al Madani, S. T. Hall, M. Gleeson, R. J. Scott, J. Roberts-Thomson, D. M. Weir, and A. Busuttil Cytokine responses and sudden infant death syndrome: genetic, developmental, and environmental risk factors J. Leukoc. Biol., December 1, 2005; 78(6): 1242 - 1254. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
