AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol (July 3, 2003). doi:10.1152/ajpgi.00042.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/5/G898    most recent
00042.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bernotti, S.
Right arrow Articles by Levy, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bernotti, S.
Right arrow Articles by Levy, E.
Submitted on January 24, 2003
Accepted on June 20, 2003

INFLAMMATORY REACTION WITHOUT ENDOGENOUS ANTIOXIDANT RESPONSE IN CACO-2 CELLS EXPOSED TO IRON / ASCORBATE-MEDIATED LIPID PEROXIDATION

Sandra Bernotti1, Ernest Seidman2, Daniel Sinnett3, Sylvain Brunet1, Serge Dionne4, Edgard Delvin5, and Emile Levy1*

1 Centre de Recherche, Hopital Sainte-Justine, Montreal, Quebec, Canada; Department of Nutrition, Universite de Montreal, Montreal, Quebec, Canada
2 Centre de Recherche, Hopital Sainte-Justine, Montreal, Quebec, Canada; Department of Pediatrics, Universite de Montreal, Montreal, Quebec, Canada
3 Centre de Recherche, Hopital Sainte-Justine, Montreal, Quebec, Canada; Department of Pediatrics, Universite de Montreal, Montreal, Quebec, Canada; Department of Biochemistry, Universite de Montreal, Montreal, Quebec, Canada
4 Centre de Recherche, Hopital Sainte-Justine, Montreal, Quebec, Canada
5 Centre de Recherche, Hopital Sainte-Justine, Montreal, Quebec, Canada; Department of Biochemistry, Universite de Montreal, Montreal, Quebec, Canada

* To whom correspondence should be addressed. E-mail: levye{at}justine.umontreal.ca.

To characterize the role of intestinal epithelial cells in mucosal host defense, we have examined endogenous antioxidant reactivity and inflammatory response in Caco-2 cell line. When differentiated Caco-2 cells were incubated with iron/ascorbate for 1-24h, they exhibited increased MDA levels and decreased polyunsaturated fatty acid proportion in favor of saturated fatty acids. These modifications were accompanied with alterations in membrane fluidity and permeability. The oxidative stress did not induce changes in the antioxidant enzyme activity of superoxide dismutase, catalase, glutathione peroxidase and glutathione transferase, or in cellular glutathione content. However, iron/ascorbate-mediated lipid peroxidation promoted I{kappa}B degradation and NF-{kappa}B activation, as well as gave rise to interleukin-8, COX-2 and ICAM-1. These results support the importance of oxidant/antioxidant balance in the epithelial cell inflammatory response.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Levy, K. Trudel, M. Bendayan, E. Seidman, E. Delvin, M. Elchebly, J.-C. Lavoie, L.-P. Precourt, D. Amre, and D. Sinnett
Biological role, protein expression, subcellular localization, and oxidative stress response of paraoxonase 2 in the intestine of humans and rats
Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1252 - G1261.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Song, J. Li, A. Lulla, B. M. Evers, and D. H. Chung
Protein kinase D protects against oxidative stress-induced intestinal epithelial cell injury via Rho/ROK/PKC-{delta} pathway activation
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1469 - C1476.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1979 by the American Physiological Society.