The aim of this study was to investigate effects of synchronized intestinal electrical stimulation (SIES) on small intestinal motility in dogs. Seventeen dogs were equipped with a duodenal cannula for the measurement of small bowel motility using manometry; an additional cannula was equipped in six of the dogs with 1.5 m distal to the first one for the measurement of small intestinal transit. Two pairs of bipolar electrodes were implanted on the small intestinal serosa with an interval of 5cm; Glucagon was used to induce postprandial intestinal hypomotility. Eleven dogs were used for the assessment of the small intestinal contractions in both fasting and fed states. The other six dogs were used for the measurement of small intestinal transit. We found: 1) SIES induced small intestinal contractions during phase I of the migrating motor complex. (Contractile index or CI: 5.2 ± 0.6 vs. 10.3 ± 0.7, P = 0.003) 2). In the fed state, SIES significantly improved glucagon-induced small intestinal postprandial hypomotility (CI: 3.4 ± 0.5 vs. 6.0 ± 0.3, P = 0.03) 3). SIES significantly accelerated small intestinal transit delayed by glucagon (70.4 ± 3.1 min vs. 44.5 ± 3.1 min, P <0.01). 4). There was a negative correlation between the contractile index and transit time (r=-0.427, p=0.048). 5).The excitatory effect of SIES was blocked by atropine. SIES may have a therapeutic potential for treating patients with small intestinal disorders.