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Am J Physiol Gastrointest Liver Physiol (October 9, 2002). doi:10.1152/ajpgi.00093.2002
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Articles in PresS, published online ahead of print October 9, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00093.2002
Submitted on March 8, 2002
Accepted on October 1, 2002

Local presentation of Steel factor increases expression of cKit immunoreactive interstitial cells of Cajal in culture

Adam Rich1*, Steven M. Miller1, Simon J. Gibbons2, John Malysz1, Joseph H. Szurszewski2, and Gianrico Farrugia2

1 Enteric NeuroScience Program, Mayo Clinic and Mayo Foundation, Rochester, MN, USA; Department of Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, MN, USA
2 Enteric NeuroScience Program, Mayo Clinic and Mayo Foundation, Rochester, MN, USA; Department of Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, MN, USA; Division of Gastroenterology and Hepatology, Mayo Clinic and Mayo Foundation, Rochester, MN, USA

* To whom correspondence should be addressed. E-mail: adam.rich{at}bms.com.

The binding of Steel factor (SF) to c-kit initiates a signaling pathway that is essential for development and maintenance of interstitial cells of Cajal (ICC). Soluble and membrane bound isoforms of SF are expressed in the gastrointestinal tract but the role for either isoform in supporting ICC development is unknown. The aim of this study was to determine the role of SF in supporting ICC in culture. ICC were cultured from dissociated mouse jejunum and grown with fibroblast cell lines that produced either soluble, membrane-bound or membrane restricted SF. ICC were identified and counted by c-kit immunoreactivity. The number of c-kit immunoreactive cells was greater in the co-culture system as compared to cultures grown without SF producing fibroblasts. All forms of SF-producing fibroblasts increased ICC number in culture but physical separation of the fibroblasts from the c-kit immunoreactive cells, addition of exogenous SF to the culture medium, or fibroblast-conditioned media did not. These results are consistent with the hypothesis that the membrane-bound form of SF preferentially contributes to expression of c-kit positive ICC under cell culture conditions.




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