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1 Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States
2 Department of Pediatrics, Baylor College of Medicine, Houston, Texas, United States
3 Department of Pathology, Baylor College of Medicine, United States
4 Houston, Texas, United States; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States
5 Departments of Pediatrics and Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States
* To whom correspondence should be addressed. E-mail: shenning{at}bcm.tmc.edu.
Although glucocorticoids are known to elicit functional maturation of the gastrointestinal tract, the molecular mechanisms of glucocorticoid action on the developing intestine have not been fully elucidated. Our previous microarray studies identified 66 transcripts as being rapidly induced in the jejunum following dexamethasone (DEX) administration to suckling mice. Now we report the specific cellular location of a subset of these transcripts. Mouse pups at P8 received DEX or vehicle and intestinal segments were collected 3-4 h later. Robotic-based in situ hybridization (ISH) was performed with digoxygenin-labeled riboprobes. Transcripts studied included: Ndrg1, Sgk1, Fos and two unknown genes (Gene 9 and Gene 36). As predicted, ISH revealed marked diversity of cellular expression. In small intestinal segments: Sgk1 mRNA was in all epithelial cells; Fos mRNA was confined to epithelial cells at the villus tip and Ndrg1 and Gene 36 mRNAs were localized to epithelial cells of the upper crypt and villus base. The remaining transcript (Gene 9) was induced modestly in villus stroma and strongly in the muscle layers. In the colon: Ndrg1, Sgk1 and Gene 36 were induced in all epithelial cells; Gene 9 in muscle layers only and Fos was not detectable. For jejunal segments, quantitation of ISH signals in tissue from DEX-treated and vehicle-treated mice demonstrated mRNA increases very similar to those measured by Northern blotting. We conclude that glucocorticoid action in the intestine reflects diverse molecular mechanisms operating in different cell types and that quantitative ISH is a valuable tool for studying hormone action in this tissue.
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