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Am J Physiol Gastrointest Liver Physiol (December 4, 2002). doi:10.1152/ajpgi.00172.2002
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Articles in PresS, published online ahead of print December 4, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00172.2002
Submitted on May 13, 2002
Accepted on November 29, 2002

Cyclooxygenase Blockade and Exogenous Glutamine Enhance Sodium Absorption in Infected Bovine Ileum

Jeffrey T. Cole1*, Anthony T. Blikslager2, Elaine Hunt3, Jody L. Gookin1, and Robert A. Argenzio1

1 Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, USA
2 Department of Clinical Sciences, North Carolina State University, Raleigh, NC, USA
3 Department of Food Animal Health and Resource Management, North Carolina State University, Raleigh, NC, USA

* To whom correspondence should be addressed. E-mail: Jtcole{at}unity.ncsu.edu.

We have previously shown that prostanoids inhibit electroneutral sodium absorption in Cryptosporidium parvum-infected porcine ileum, whereas glutamine stimulates electroneutral sodium absorption. We postulated that glutamine would stimulate sodium absorption via a COX-dependent pathway. We tested this hypothesis in C. parvum-infected calves, which are the natural hosts of cryptosporidiosis. Tissues from healthy and infected calves were studied in Ussing chambers, and analyzed via immunohistochemistry and Western blots. Treatment of infected tissue with selective COX inhibitors revealed that COX-1 and COX-2 must be blocked to restore electroneutral sodium absorption, although the transporter involved did not appear to be the expected NHE-3 isoform. Glutamine addition also stimulated sodium absorption in calf tissue, but although this transport was electroneutral in healthy tissue, sodium absorption was electrogenic in infected tissue, and was additive to sodium transport uncovered by COX inhibition. Blockade of both COX isoforms is necessary to release the prostaglandin-mediated inhibition of electroneutral sodium uptake in C. parvum infected calf ileal tissue, whereas glutamine increases sodium uptake by an electrogenic mechanism in this same tissue.




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