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1 Department of Pediatrics and Physiology, University of Arizona Health Sciences Center, Steele Memorial Children's Research Center, Tucson, AZ, USA
* To whom correspondence should be addressed. E-mail: fghishan{at}peds.arizona.edu.
The current experiments were designed to study the effect of 
-estradiol (E2) on NaPi-IIb gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by ~ 45% in rat intestine. Northern blotting indicated that NaPi-IIb mRNA expression was increased by ~ 50% after estrogen treatment. Western blot analysis also detected an increase in BBM NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased ~ 60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorption in female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus, the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription.
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