Submitted on May 24, 2006
Accepted on June 14, 2006
Rat liver endothelial cells isolated by anti-CD31 immunomagnetic separation lack fenestrae and sieve plates
Laurie D. DeLeve1*, Xiangdong Wang1, Margaret M. McCuskey2, and Robert S McCuskey2
1 Division of Gastrointestinal and Liver Diseases and the Research Center for Liver Diseases, University of Southern California Keck School of Medicine, Los Angeles, California, United States
2 Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona, United States
* To whom correspondence should be addressed. E-mail: deleve{at}usc.edu.
The gold standard for the identification of sinusoidal endothelial cells (SEC) is the presence of fenestrae organized in sieve plates, which is characteristic of SEC in vivo. One of the methods currently in use to isolate SEC is immunomagnetic sorting for CD31. However there is evidence to suggest that CD31 is not present on the surface of differentiated SEC. The current study used scanning electron microscopy to image rat hepatic endothelial cells isolated by anti-CD31 and immunomagnetic sorting and cells isolated by gradient centrifugation and centrifugal elutriation. Cells isolated by elutriation had well-developed fenestrae and sieve plates, whereas cells isolated by anti-CD31 and immunomagnetic sorting had significantly fewer fenestrae organized in sieve plates. In conclusion: cells isolated by anti-CD31 and immunomagnetic sorting lacked the hallmark features of SEC.
