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Am J Physiol Gastrointest Liver Physiol (January 29, 2003). doi:10.1152/ajpgi.00231.2002
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Submitted on June 17, 2002
Accepted on January 29, 2003

Glucagon-like peptide-2 enhanced barrier function reduces pathophysiology in a model of food allergy

Heather L. Cameron1, Ping-Chang Yang1, and Mary H. Perdue1*

1 Intestinal Disease Research Program, McMaster University, Hamilton, ON, Canada

* To whom correspondence should be addressed. E-mail: perdue{at}mcmaster.ca.

Penetration of the gut epithelial barrier by intact luminal antigen is necessary for immunologically-mediated pathophysiology in the context of food allergy. We investigated if glucagon-like peptide-2 (GLP-2) could affect immediate hypersensitivity and late phase allergic inflammation in a murine model. Mice were sensitized to horseradish peroxidase (HRP); studies were conducted 14 days later. Mice were treated with 5 µg of GLP-2 sc 4h before antigen challenge. For immediate hypersensitivity, jejunal segments in Ussing chambers were challenged by luminal HRP antigen. GLP-2 treatment reduced the uptake of HRP and the antigen-induced secretory response after luminal challenge. GLP-2 appears to reduce macromolecular uptake independent of the CD23 mediated enhanced antigen uptake pathway. For the late phase, mice were gavaged with antigen and 48 h later the function and histology of the jejunum was examined. GLP-2 prevented the usual prolonged permeability defect and reduced the number of inflammatory cells in the mucosa. Our studies demonstrate that a single treatment of sensitized mice with GLP diminishes both immediate and late phase hypersensitivity reactions characteristic of food allergy by inhibiting transepithelial uptake of antigen.




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