AJP - GI Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


Am J Physiol Gastrointest Liver Physiol (October 27, 2005). doi:10.1152/ajpgi.00248.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/4/G616    most recent
00248.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sato, N.
Right arrow Articles by Kozar, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sato, N.
Right arrow Articles by Kozar, R. A.
Submitted on May 31, 2005
Accepted on October 7, 2005

DIFFERENTIAL INDUCTION OF PPAR{gamma} BY LUMINAL GLUTAMINE AND iNOS BY LUMINAL ARGININE IN THE RODENT POST ISCHEMIC SMALL BOWEL

N. Sato1, F. A. Moore1, B. C. Kone2, L. Zou2, M. A. Smith1, M. A. Childs1, Olufemi-Moore1, S. G. Schultz3, and R. A. Kozar1*

1 Department of Surgery, University of Texas-Houston School of Medicine, Houston, TX, USA
2 Department of Medicine, University of Texas-Houston School of Medicine, Houston, TX, USA
3 Department of Integrative Biology and Pharmacology, University of Texas-Houston School of Medicine, Houston, TX, USA

* To whom correspondence should be addressed. E-mail: Rosemary.A.Kozar{at}uth.tmc.edu.

Using a rodent model of gut ischemia/ reperfusion (IR), we have previously shown that the induction of iNOS is harmful while the induction of HO-1 and PPAR-gamma is protective. In the present study, we hypothesized that the luminal nutrients, arginine and glutamine, differentially modulate these molecular events in the post ischemic gut. Jejunal sacs were created in rats at laparotomy and filled with either 60 mM glutamine, arginine, or magnesium sulfate (osmotic control) followed by 60 minutes of superior mesenteric artery occlusion and 6 hours of reperfusion and compared to shams. Jejunum was harvested for histology or myeloperoxidase activity (MPO, inflammation). Heat shock proteins and iNOS were quantitated by Western Blot analysis and PPAR{gamma} by DNA binding activity. In some experiments, rats were pretreated with the PPAR{gamma} inhibitor, G9662, or with the iNOS inhibitor,1400W. iNOS, was significantly increased by arginine but not glutamine following gut IR and was associated with increased MPO activity and mucosal injury. On the other hand, PPAR{gamma}, was significantly increased by glutamine but decreased by arginine while heat shock proteins were similarly increased in all experimental groups. The PPAR{gamma} inhibitor, G9662, abrogated the protective effects of glutamine while the iNOS inhibitor,1400W, attenuated the injurious effects of arginine. We concluded that luminal arginine and glutamine differentially modulate the molecular events that regulate injurious IR-mediated gut inflammation and injury. The induction of PPAR{gamma} by luminal glutamine is a novel protective mechanism while luminal arginine appears harmful to the post ischemic gut due to enhanced expression of iNOS.




This article has been cited by other articles:


Home page
Nutr Clin PractHome page
F. A. Moore and E. E. Moore
The Evolving Rationale for Early Enteral Nutrition Based on Paradigms of Multiple Organ Failure: A Personal Journey
Nutr Clin Pract, June 1, 2009; 24(3): 297 - 304.
[Full Text] [PDF]


Home page
GutHome page
R Marion-Letellier, P Dechelotte, M Iacucci, and S Ghosh
Dietary modulation of peroxisome proliferator-activated receptor gamma
Gut, April 1, 2009; 58(4): 586 - 593.
[Abstract] [Full Text] [PDF]


Home page
J. Leukoc. Biol.Home page
K. Ban and R. A. Kozar
Enteral glutamine: a novel mediator of PPAR{gamma} in the postischemic gut
J. Leukoc. Biol., September 1, 2008; 84(3): 595 - 599.
[Abstract] [Full Text] [PDF]


Home page
JPEN J Parenter Enteral NutrHome page
E. K. Robinson, D. P. Kelly, D. W. Mercer, and R. A. Kozar
Differential Effects of Luminal Arginine and Glutamine on Metalloproteinase Production in the Postischemic Gut
JPEN J Parenter Enteral Nutr, July 1, 2008; 32(4): 433 - 438.
[Abstract] [Full Text] [PDF]


Home page
JPEN J Parenter Enteral NutrHome page
M. McQuiggan, R. Kozar, R. M. Sailors, C. Ahn, B. McKinley, and F. Moore
Enteral Glutamine During Active Shock Resuscitation Is Safe and Enhances Tolerance of Enteral Feeding
JPEN J Parenter Enteral Nutr, January 1, 2008; 32(1): 28 - 35.
[Abstract] [Full Text] [PDF]


Home page
GutHome page
L Dubuquoy, C Rousseaux, X Thuru, L Peyrin-Biroulet, O Romano, P Chavatte, M Chamaillard, and P Desreumaux
PPAR{gamma} as a new therapeutic target in inflammatory bowel diseases.
Gut, September 1, 2006; 55(9): 1341 - 1349.
[Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.