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Am J Physiol Gastrointest Liver Physiol (October 30, 2003). doi:10.1152/ajpgi.00261.2003
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Submitted on June 16, 2003
Accepted on October 23, 2003

Phosphorylated HSP27 essential for Acetylcholine-Induced association of RhoA with PKC{alpha}

Suresh B. Patil1, Mercy D Pawar1, and Khalil N. Bitar1*

1 Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA

* To whom correspondence should be addressed. E-mail: bitar{at}umich.edu.

Reorganization of the cytoskeleton and association of contractile proteins are important steps in modulating smooth muscle contraction. HSP27 has significant effects on actin cytoskeletal reorganization during smooth muscle contraction. We investigated the role of phosphorylated HSP27 in modulating acetylcholine-induced sustained contraction of smooth muscle cells from the colon by transfecting smooth muscle cells with phospho-mimic (3D) or non-phospho- mimic (3G) HSP27. In 3G cells, the initial peak contractile response at 30 sec was inhibited by 25 % (24.0 ± 4.5 % decrease in cell length, n=4). The sustained contraction was greatly inhibited by 75% (9.3 ±.9 % decreases in cell length (n=4). Further, in 3D cells translocation of both PKC{alpha} and of RhoA was greatly enhanced and resulted in a greater association of PKC{alpha}-RhoA in the membrane fraction. In 3G transfected cells, PKC{alpha} and RhoA failed to translocate in response to stimulation with acetylcholine, resulting in an inhibition of association of PKC{alpha}-RhoA in the membrane fraction. Studies using GST-RhoA fusion protein indicate that there is a direct association of RhoA with PKC{alpha} and with HSP27. The results suggest that phosphorylated HSP27 plays a crucial role in the maintenance of association of PKC{alpha}-RhoA in the membrane fraction, and in the maintenance of acetylcholine-induced sustained contraction.




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