| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print September 21, 2001
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00263.2001
Submitted on June 18, 2001
Accepted on September 4, 2001
1 Medicine, Division of Gastroenterology, University of Pittsburgh, Pittsburgh, PA, USA
2 Surgery, Rheinische Friedrich-Wilhelms-Universitat, Bonn, NRW, Germany
* To whom correspondence should be addressed. E-mail: tbauer{at}pitt.edu.
Endotoxemia causes an inflammatory response within the intestinal muscularis and gastrointestinal dysmotility. We hypothesize that the resident macrophage-derived chemokine MCP-1 plays a significant role in the recruitment of leukocytes into the LPS stimulated rat intestinal muscularis. MCP-1 mRNA expression was investigated by RT?PCR. Leukocyte extravasation and MCP-1 protein localization were determined by immunohistochemistry. Contractile activity was assessed using a standard organ bath in rats that were treated with saline, rMCP-1, LPS, LPS + non-specific antibody or LPS + MCP-1 antibody. Endotoxemia caused a significant 280-fold increase in MCP-1 mRNA expression in the muscularis, peaking at 3 hours. MCP-1 protein was immunohistochemically located to muscularis macrophages. LPS application caused significant leukocyte recruitment into the muscularis and a 51% decrease in muscle contractility. MCP-1 antibody treatment significantly averted leukocyte recruitment and significantly prevented muscle dysfunction. These parameters were not significantly altered by the non-specific antibody. Results show that resident muscularis macrophage-derived MCP-1 plays a major role in the recruitment of monocytes during endotoxemia, which then subsequently secrete kinetically active substances that cause ileus.
This article has been cited by other articles:
|
|
 |
|
  M. Hori, H. Nobe, K. Horiguchi, and H. Ozaki MCP-1 targeting inhibits muscularis macrophage recruitment and intestinal smooth muscle dysfunction in colonic inflammation Am J Physiol Cell Physiol, February 1, 2008; 294(2): C391 - C401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Moore, K. M. Albers, B. M. Davis, J. R. Grandis, S. Togel, and A. J. Bauer Altered inflammatory gene expression underlies increased susceptibility to murine postoperative ileus with advancing age Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1650 - G1659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohama, M. Hori, E. Momotani, M. Elorza, W. T. Gerthoffer, and H. Ozaki IL-1beta inhibits intestinal smooth muscle proliferation in an organ culture system: involvement of COX-2 and iNOS induction in muscularis resident macrophages Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1315 - G1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hamano, T. Inada, R. Iwata, T. Asai, and K. Shingu The {alpha}2-adrenergic receptor antagonist yohimbine improves endotoxin-induced inhibition of gastrointestinal motility in mice Br. J. Anaesth., April 1, 2007; 98(4): 484 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wehner, F. F Behrendt, B. N Lyutenski, M. Lysson, A. J Bauer, A. Hirner, and J. C Kalff Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents Gut, February 1, 2007; 56(2): 176 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Overhaus, B. A. Moore, J. E. Barbato, F. F. Behrendt, J. G. Doering, and A. J. Bauer Biliverdin protects against polymicrobial sepsis by modulating inflammatory mediators Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G695 - G703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Mythen Postoperative Gastrointestinal Tract Dysfunction Anesth. Analg., January 1, 2005; 100(1): 196 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Watanabe, K. Higuchi, M. Hamaguchi, M. Shiba, K. Tominaga, Y. Fujiwara, T. Matsumoto, and T. Arakawa Monocyte chemotactic protein-1 regulates leukocyte recruitment during gastric ulcer recurrence induced by tumor necrosis factor-{alpha} Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G919 - G928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Overhaus, S. Togel, M. A. Pezzone, and A. J. Bauer Mechanisms of polymicrobial sepsis-induced ileus Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G685 - G694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ramani, M. Mathier, P. Wang, G. Gibson, S. Togel, J. Dawson, A. Bauer, S. Alber, S. C. Watkins, C. F. McTiernan, et al. Inhibition of tumor necrosis factor receptor-1-mediated pathways has beneficial effects in a murine model of postischemic remodeling Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1369 - H1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Moore, A. Turler, M. A. Pezzone, K. Dyer, J. Grandis, and A. J. Bauer Tyrphostin AG 126 inhibits development of postoperative ileus induced by surgical manipulation of murine colon Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G214 - G224. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
