| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print December 19, 2001
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00269.2001
Submitted on June 21, 2001
Accepted on December 18, 2001
1 Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA
2 Graduate Program in Nutrition, University of North Carolina at Greensboro, Greensboro, NC, USA
3 Mineral Bioavailability Laboratory, Tufts University, Boston, MA, USA
* To whom correspondence should be addressed. E-mail: fleetj{at}cfs.purdue.edu.
The parental cell line (P) of Caco-2 cells and two clones, BBe and TC7, were studied at 11 days post-confluence to test the facilitated diffusion model of vitamin D-mediated, intestinal calcium absorption (CaTx). Nuclear vitamin D receptor (nVDR) and calbindin D9k (CaBP) were measured by Western blot, 24-hydroxylase (CYP24), CaBP, Ca ATPase (PMCA), and Ca channel (CaT1) mRNA levels were examined by RT-PCR, and net apical-to-basolateral CaTx was examined after treating cells for +/- 10 nM calcitriol for 8 hours (mRNA levels) or 48 hours (protein, CaBP mRNA, CaTx). nVDR level was lowest in BBe (38% P value) and directly related to CYP24 induction (TC7 = P 1.56X > BBe). nVDR was inversely related to the vitamin D-induced levels of CaT1 mRNA, CaBP mRNA, PMCA mRNA, and net CaTx with the highest induction seen in BBe. Basal CaBP mRNA (86X>P) and protein levels were highest in TC7 cells and were not associated with higher net CaTx suggesting CaBP may not be rate limiting for CaTx in these cells.
This article has been cited by other articles:
|
|
 |
|
  N. Thongon, L.-i. Nakkrasae, J. Thongbunchoo, N. Krishnamra, and N. Charoenphandhu Prolactin stimulates transepithelial calcium transport and modulates paracellular permselectivity in Caco-2 monolayer: mediation by PKC and ROCK pathways Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1158 - C1168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Jantarajit, N. Thongon, J. Pandaranandaka, J. Teerapornpuntakit, N. Krishnamra, and N. Charoenphandhu Prolactin-stimulated transepithelial calcium transport in duodenum and Caco-2 monolayer are mediated by the phosphoinositide 3-kinase pathway Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E372 - E384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels Calcium Absorption Across Epithelia Physiol Rev, January 1, 2005; 85(1): 373 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, A. Klopot, J.-N. Freund, L. N. Dowling, S. D. Krasinski, and J. C. Fleet Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1{alpha} Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G943 - G953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Wood, L. Tchack, G. Angelo, R. E. Pratt, and L. A. Sonna DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line Physiol Genomics, April 13, 2004; 17(2): 122 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-B. Peng, E. M Brown, and M. A Hediger Epithelial Ca2+ entry channels: transcellular Ca2+ transport and beyond J. Physiol., September 15, 2003; 551(3): 729 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-B. Peng, E. M. Brown, and M. A. Hediger Apical Entry Channels in Calcium-Transporting Epithelia Physiology, August 1, 2003; 18(4): 158 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yang, Y. Fan, and D. H. Teitelbaum Intraepithelial lymphocyte-derived interferon-gamma evokes enterocyte apoptosis with parenteral nutrition in mice Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G629 - G637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Fleet, L. Wang, O. Vitek, B. A. Craig, and H. J. Edenberg Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation Physiol Genomics, March 18, 2003; 13(1): 57 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Song, S. Kato, and J. C. Fleet Vitamin D Receptor (VDR) Knockout Mice Reveal VDR-Independent Regulation of Intestinal Calcium Absorption and ECaC2 and Calbindin D9k mRNA J. Nutr., February 1, 2003; 133(2): 374 - 380. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
