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1 Department of Physiology, School of Veterinary Medicine, Hannover, Germany
2 Department of Veterinary Physiology, Free University of Berlin, Berlin, Germany
* To whom correspondence should be addressed. E-mail: sabine.leonhard-marek{at}tiho-hannover.de.
High potassium diets lead to an inverse regulation of sodium and magnesium absorption in ruminants, suggesting some form of cross-talk. Previous Ussing chamber experiments have demonstrated a divalent sensitive Na+ conductance in the apical membrane of ruminal epithelium. Using patch-clamped ruminal epithelial cells, we could observe a divalent sensitive, non-selective cation conductance (NSCC) with PK>PCs>PNa. Conductance increased and rectification decreased when either Mg2+, or both Ca2+ and Mg2+ were removed from the internal or external solution or both. The conductance could be blocked by Ba2+, but not by TEA. Subsequently, we studied this conductance measured as short circuit current (ISC) in Ussing chambers. Forskolin, IBMX and theophylline are known to block both ISC and Na transport across ruminal epithelium in the presence of divalent cations. When the NSCC was stimulated by removing mucosal calcium, an initial decrease in ISC was followed by a subsequent increase. The cAMP mediated increase in ISC was reduced by low serosal Na+, serosal addition of imipramine or serosal amiloride, and depended upon the availability of mucosal magnesium. Luminal amiloride had no effect. Flux studies showed that low serosal Na+ reduced 28Mg fluxes from mucosal to serosal. The data suggest that cAMP stimulates basolateral Na+/Mg2+ exchange, reducing cytosolic Mg. This increases sodium uptake through a magnesium-sensitive NSCC in the apical membrane. Likewise, the reduction in magnesium uptake that follows ingestion of high potassium fodder may facilitate sodium absorption, as observed in studies of ruminal osmoregulation. Possibly, grass tetany (hypomagnesaemia) is a side-effect of this useful mechanism.
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