Background: Children with cholestatic liver diseases, in particular Biliary Atresia (BA), may develop an acquired growth hormone (GH) resistance. This is characterized by normal GH secretion, reduced liver GH receptor (GHR) abundance, and reduced circulating Insulin-Like Growth Factor 1 (IGF-1). Consequences include linear growth failure, reduced muscle mass, and increased perioperative morbidity and mortality. However, the molecular basis for altered GH signaling in liver and skeletal muscle in cholestatic liver disease is not known. We hypothesized that reduced IGF-1 expression in obstructive cholestasis would be associated with down regulation of the GHR and impaired phosphorylation of STAT5 transcription factors. Methods: Body composition was determined in C57BL/6J male mice after bile duct ligation (BDL) relative to pair fed (PF) and Ad Lib (AL) fed controls. GHR, STAT5, Sp3, and IGF-1 expression and/or DNA binding were assessed using immunoblots (IB), electrophoretic mobility shift assays (EMSA), and/or real time RT-PCR. Results: Fat free mass (FFM) was reduced in PF mice relative to AL fed controls. BDL led to a further reduction in fat mass (FM) and FFM relative to PF controls. TNF was increased in liver and skeletal muscle of BDL mice. This was associated with reduced GH dependent STAT5 activation and IGF-1 RNA expression. GHR expression was reduced in BDL mice; in liver this was associated with reduced Sp3 binding to a GHR gene promoter cis element. Conclusions: Wasting in murine obstructive cholestasis is due to combined effects of reduced caloric intake and biliary obstruction. Growth hormone resistance due to down regulation of GHR expression may be attributed primarily to the obstructive cholestasis; therapies which specifically increase GHR expression may restore GH signaling in this setting.