AJP - GI AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol (November 21, 2001). doi:10.1152/ajpgi.00291.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/G508    most recent
00291.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ko, W. H.
Right arrow Articles by Huang, Y.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ko, W. H.
Right arrow Articles by Huang, Y.

Articles in PresS, published online ahead of print November 21, 2001
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00291.2001
Submitted on July 13, 2001
Accepted on November 16, 2001

STIMULATION OF CHLORIDE SECRETION BY BAICALEIN IN ISOLATED RAT DISTAL COLON

Wing Hung Ko1*, Vincent W Law1, Wallace C Yip1, Grace G Yue1, Chi Wai Lau1, Zhen Yu Chen2, and Yu Huang1

1 Physiology, The Chinese University of Hong Kong, Shatin, Hong Kong
2 Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong

* To whom correspondence should be addressed. E-mail: whko{at}cuhk.edu.hk.

The effect of baicalein on mucosal ion transport in the rat distal colon was investigated in Ussing chambers. Mucosal addition of baicalein (1-100 µM) elicited a concentration-dependent ISC response. The increase in ISC was mainly due to Cl- secretion. The presence of mucosal indomethacin (10 µM) significantly reduced both the basal and subsequent baicalein-evoked ISC responses. The baicalein-induced ISC were inhibited by basolateral application of chromanol 293B (30µM), a blocker of KvLQT1 channels and Ba2+ ions (5mM). Treatment of the colonic mucosa with baicalein elicited a three-fold increase in cAMP production. Pretreating the colonic mucosa with carbachol (100 µM, serosal) but not thapsigargin (1 µM, both sides) abolished the baicalein-induced ISC. Addition of baicalein subsequent to forskolin induced a further increase in ISC. These results indicate that the baicalein-evoked Cl- secretion across rat colonic mucosa, possibly via cAMP-dependent pathway. However, the action of baicalein cannot be solely explained by its cAMP-elevating effect. Baicalein may stimulate Cl- secretion via a cAMP-independent pathway or have a direct effect on CFTR.




This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
D. Wu and Z. Hu
Rutaecarpine Induces Chloride Secretion across Rat Isolated Distal Colon
J. Pharmacol. Exp. Ther., April 1, 2008; 325(1): 256 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
I. Blumenstein, B. Keseru, F. Wolter, and J. Stein
The Chemopreventive Agent Resveratrol Stimulates Cyclic AMP-Dependent Chloride Secretion In vitro
Clin. Cancer Res., August 1, 2005; 11(15): 5651 - 5656.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Cavelier and D. Attwell
Tonic release of glutamate by a DIDS-sensitive mechanism in rat hippocampal slices
J. Physiol., April 15, 2005; 564(2): 397 - 410.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1979 by the American Physiological Society.