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Articles in PresS, published online ahead of print October 3, 2001
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00319.2001
Submitted on July 24, 2001
Accepted on September 21, 2001
* To whom correspondence should be addressed. E-mail: fghishan{at}peds.arizona.edu.
We sought to characterize expression of an apically expressed intestinal sodium-phosphate cotransporter (Na/Pi-IIb) during mouse ontogeny and to assess the effects of methylprednisolone (MP) treatment. In control mice, sodium-dependent Pi (Na/Pi) uptake by intestinal brush-border membrane vesicles was highest at 14-days-of-age, lower at 21 days and further reduced at 8 weeks and 8-9 months of age. Na/Pi-IIb mRNA and immunoreactive protein levels in 14-d animals were markedly higher than in older groups. MP treatment significantly decreased Na/Pi uptake, and Na/Pi-IIb mRNA and protein expression in 14-d mice. Additionally, the size of the protein was smaller in 14-d mice. Deglycosylation of protein from 14-d and 8-wk old animals with PNGase reduced the molecular weight to the predicted size. We conclude that intestinal Na/Pi uptake and Na/Pi-IIb expression are highest at 14-d and decrease with age. Furthermore, MP treatment reduced intestinal Na/Pi uptake ~3-fold in 14-d mice and this reduction correlates with reduced Na/Pi-IIb mRNA and protein expression. We also demonstrate that Na/Pi-IIb is an N-linked glycoprotein and that glycosylation is age-dependent.
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