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Am J Physiol Gastrointest Liver Physiol (February 20, 2002). doi:10.1152/ajpgi.00320.2001
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Articles in PresS, published online ahead of print February 20, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00320.2001
Submitted on July 20, 2001
Accepted on February 4, 2002

Intestinal inflammation modulates expression of the synaptic vesicle protein Neuronal Calcium Sensor (NCS-1)

Sandra Lourenssen, Andreas Jeromin, John Roder, and Michael G Blennerhassett*

* To whom correspondence should be addressed. E-mail: mblen{at}meds.queensu.ca.

The calcium-binding protein neuronal calcium sensor 1 (NCS-1) is involved in modulation of neurotransmitter release in the peripheral and central nervous systems. Since intestinal inflammation impairs neurotransmitter release, we evaluated the expression of NCS-1 in the normal rat colon and in dinitrobenzenesulphonic acid (DNBS)-induced colitis. Immunocytochemistry and Western blots showed high levels of NCS-1 in the myenteric plexus and in axons in the smooth muscle layers. 23 ± 2% of myenteric neurons were NCS-1 positive, with staining restricted to the largest neurons. NCS-1 positive axons decreased to 13.3 ± 0.4% of total axons by Day 2, and dropped further to 7.0 ± 0.1% by Day 4, returning to control levels by Day 16. Dual label Western blot analysis showed that the expression of NCS-1 relative to PGP 9.5 decreased by 50% on Day 4, but returned to control by Day 16. The selective loss of NCS-1 during colitis may underlie the altered neural function seen in the inflamed intestine.




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