AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol (January 15, 2003). doi:10.1152/ajpgi.00326.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/5/G853    most recent
00326.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hinojosa-Kurtzberg, A. M.
Right arrow Articles by Gendler, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hinojosa-Kurtzberg, A. M.
Right arrow Articles by Gendler, S. J.
Submitted on August 6, 2002
Accepted on January 8, 2003

Novel MUC1 splice variants contribute to mucin over-expression in CFTR deficient mice

A. Marina Hinojosa-Kurtzberg1, Malin E. V. Johansson2, Cathy S. Madsen1, Gunnar C. Hansson2, and Sandra J. Gendler1*

1 Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Scottsdale, AZ, USA
2 Department of Medical Biochemistry, Goteborg University, 413 90 Gothenburg, Sweden

* To whom correspondence should be addressed. E-mail: gendler.sandra{at}mayo.edu.

A CF mouse expressing the human MUC1 transgene (CFM mice) reverted the CF/Muc1-/- phenotype (little mucus accumulated in the intestine) to that of CF mice expressing mouse Muc1, which exhibited increased mucus accumulation. Western blots and immunohistochemical analysis showed that the MUC1 protein was markedly increased in CFM mice where it was both membrane bound and secreted into the intestinal lumen. Studies to determine the reason for increased levels of the extracellular domain of MUC1 mucin identified mRNA and protein of two novel splice variants and the previously described splice variant MUC1/SEC (lacking the cytoplasmic tail). The novel MUC1 splice variants, CT80 and CT58, were both transmembrane proteins with cytoplasmic tails different from the normal MUC1. The MUC1-CT80 and MUC1/SEC forms are expressed mainly in the CFM intestines. Thus, MUC1 expression is increased and it appears that alternate cytoplasmic tails may change its role in signaling. MUC1 could be an important contributor to the CF intestinal phenotype.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. C. De Lisle, E. Roach, and K. Jansson
Effects of laxative and N-acetylcysteine on mucus accumulation, bacterial load, transit, and inflammation in the cystic fibrosis mouse small intestine
Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G577 - G584.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Kuver, T. Wong, J. H. Klinkspoor, and S. P. Lee
Absence of CFTR is associated with pleiotropic effects on mucins in mouse gallbladder epithelial cells
Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1148 - G1154.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. K. Malmberg, K. A. Noaksson, M. Phillipson, M. E. V. Johansson, M. Hinojosa-Kurtzberg, L. Holm, S. J. Gendler, and G. C. Hansson
Increased levels of mucins in the cystic fibrosis mouse small intestine, and modulator effects of the Muc1 mucin expression
Am J Physiol Gastrointest Liver Physiol, August 1, 2006; 291(2): G203 - G210.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1979 by the American Physiological Society.