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Am J Physiol Gastrointest Liver Physiol (April 17, 2002). doi:10.1152/ajpgi.00343.2001
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Articles in PresS, published online ahead of print April 17, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00343.2001
Submitted on August 9, 2001
Accepted on April 15, 2002

Mesenteric Afferent Nerves are Sensitive to Vascular Perfusion in a Novel Preparation of Rat Ileum in vitro

Alan M Brunsden1*, Samuel Jacob1, Karnadev D Bardhan2, and David Grundy1

1 Department of Biomedical Science, Sheffield University, Sheffield, United Kingdom
2 Department of Gastroenterology, Rotherham District Hospital, Rotherham, United Kingdom

* To whom correspondence should be addressed. E-mail: S.jacob{at}sheffield.ac.uk.

Using novel in vitro preparations of vascularly perfused rat ileum, we investigated mesenteric afferent sensitivity to vascular perfusion. Gut (GPP) and vascular (VPP) perfusion pressures were recorded simultaneously with afferent discharge (AD). After pre-constriction (L-phenylephrine), luminal capsaicin (100µM, gut lumen) caused a transient increase in AD and a sustained fall in VPP, supporting afferent modulation of vascular tone. In turn, AD was affected by vascular perfusion rate (VPR). Increasing VPR step-wise (0.6 to 1.0, 1.4 and 1.8ml/min), caused concomitant falls in AD, returning at 0.6ml/min. Terminating flow (5min) increased AD. Afferent responses were independent of changes in GPP, vascular O2 or the gut 'tube' (gut-off). In 'gut-off' studies, where capsaicin (100nM, i.a.) still reduced VPP, flow-associated falls in AD were abolished by the enzyme neuraminidase (0.2U/ml, 20min, i.a. or extravascular). In contrast, increased AD following stopped flow was unaffected. We propose that mesenteric afferents 'sense' changes in vascular perfusion. The precise stimuli (pressure and/or flow) and the physiological relevance to control of local circulation remain to be determined.




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