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Am J Physiol Gastrointest Liver Physiol (February 19, 2004). doi:10.1152/ajpgi.00347.2003
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Submitted on August 11, 2003
Accepted on February 3, 2004

Generation and characterization of immortalized rat pancreatic stellate cells

Gisela Sparmann1*, Christine Hohenadl2, Jens Tornoe3, Robert Jaster1, Brit Fitzner1, Dirk Koczan4, Hans-Jurgen Thiesen4, Anne Glass5, David Winder2, Stefan Liebe1, and Jorg Emmrich1

1 Department of Gastroenterology, University of Rostock, Rostock, Germany
2 Institute of Virology, University of Veterinary Sciences, Vienna, Austria
3 NsGene A/S, Ballerup, Denmark
4 Institute of Immunology, University of Rostock, Rostock, Germany
5 Institute of Medical Informatics and Biometry, University of Rostock, Rostock, Germany

* To whom correspondence should be addressed. E-mail: gisela.sparmann{at}med.uni-rostock.de.

Pancreatic stellate cells (PSC) are involved in, among other things, the pathogenesis of pancreatic fibrosis. Here, we present the generation of immortalized PSCs 7 and 14 days after isolation by retroviral gene transfer of the SV 40 large T antigen encoding region. Propagated cell lines (LTC-7, LTC-14) retained characteristics of primary cells in terms of morphology, responsiveness to mediators regulating cellular functions such as proliferation and expression profile of a number of investigated genes. While LTC-14 kept the morphological features of the differentiation status of the primary cells they were made of, LTC-7 appeared similar to an earlier stage. Thus, the established cell lines represent a versatile tool to investigate various aspects of PSC biology.




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