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Am J Physiol Gastrointest Liver Physiol (September 20, 2007). doi:10.1152/ajpgi.00350.2007
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Submitted on July 31, 2007
Accepted on September 19, 2007

Mechanisms of platelet and leukocyte recruitment in experimental colitis

Thorsten Vowinkel1, Katherine C. Wood2, Karen Y. Stokes2, Janice Russell2, Anitaben Tailor2, Christoph Anthoni1, Norbert Senninger3, Christian F. Krieglstein3, and D. Neil Granger2*

1 Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States; Department of General Surgery, University Hospital Munster, Munster, Germany
2 Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States
3 Department of General Surgery, University Hospital Munster, Munster, Germany

* To whom correspondence should be addressed. E-mail: dgrang{at}lsuhsc.edu.

Both leukocytes and platelets accumulate in the colonic microvasculature during experimental colitis, leading to microvascular dysfunction and tissue injury. The objective of this study was to determine whether the recruitment of leukocytes and platelets in inflamed colonic venules are co-dependent processes. The rolling and adherence of leukocytes and platelets in colonic venules of mice with dextran sodium sulfate (DSS)-induced colitis were monitored by intravital videomicroscopy. DSS elicited an increased recruitment of both rolling and adherent leukocytes and platelets. DSS colitic mice rendered thrombocytopenic with anti-platelet serum exhibited profound reductions in leukocyte adhesion. Neutropenia, induced with anti-neutrophil serum, significantly reduced the adhesion of leukocytes and the accumulation of platelet-leukocyte aggregates, while greatly enhancing the number of platelets that roll and adhere directly to venular endothelial cells. The enhanced platelet adhesion associated with neutropenia was mediated by platelet P-selectin interactions with endothelial cell PSGL-1. DSS colitis was also associated with an increased expression of PSGL-1 in the colonic vasculature. These findings indicate that the recruitment of leukocytes and platelets in inflamed colonic venules are co-dependent processes.




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