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Am J Physiol Gastrointest Liver Physiol (January 29, 2003). doi:10.1152/ajpgi.00383.2002
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Submitted on September 5, 2002
Accepted on January 27, 2003

Electrical stimulation in guinea-pig myenteric plexus reveals complex neuronal input and activation patterns in single ganglia

Raf Bisschops1*, Pieter Vanden Berghe1, Erwin Bellon2, Jozef Janssens1, and Jan Tack1

1 Center for Gastroenterological Research, K.U. Leuven, Leuven, Belgium
2 Medical Image Computing (Radiology - ESAT/PSI), K.U. Leuven, Leuven, Belgium

* To whom correspondence should be addressed. E-mail: raf.bisschops{at}med.kuleuven.ac.be.

The myenteric plexus plays a key role in the control of gastrointestinal motility. We used confocal calcium imaging to study responses to electrical stimulation (ETS) of interganglionic fiber tracts in entire myenteric ganglia of the guinea pig small intestine. ETS induced calcium transients in a subset of neurons: 52.2% responded to oral ETS, 65.4% to aboral ETS, 71.7% to simultaneous oral and aboral ETS. 41.3% of the neurons displayed convergence of oral and aboral ETS induced responses. Responses could be reversibly blocked with tetrodotoxin (10- 6M), demonstrating involvement of neuronal conduction, and by removal of extracellular calcium. {omega}-Conotoxin (5x10-7M) blocked the majority of responses and reduced the amplitude of residual responses by 45 %, indicating the involvement of N-type calcium channels. Staining for Calbindin and Calretinin did not reveal different response patterns in these immunohistochemically identified neurons. We conclude that at least for ETS close to a ganglion confocal calcium imaging reveals complex oral and aboral input to individual myenteric neurons, rather than a polarization in spread of activity.




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