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Am J Physiol Gastrointest Liver Physiol (April 17, 2003). doi:10.1152/ajpgi.00385.2002
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Submitted on September 6, 2002
Accepted on April 1, 2003

Enteral non-essential amino acids stimulate protein synthesis and glutamine decreases ubiquitin mRNA level in human gut mucosa

Moise Coeffier1, Sophie Claeyssens1, Bernadette Hecketsweiler1, Alain Lavoinne1, Philippe Ducrotte1, and Pierre Dechelotte1*

1 Faculte de Medecine Pharmacie, Appareil Digestif, Environnment et Nutrition (ADEN EA 3234), IFR 23, CIC-INSERM, Rouen, France

* To whom correspondence should be addressed. E-mail: pierre.dechelotte{at}chu-rouen.fr.

The effects of glutamine on whole body and intestinal protein synthesis and on intestinal proteolysis were assessed in humans. Two groups of healthy volunteers received in a random order enteral glutamine (0.8mmol.kg-1.h-1) compared either to saline, or isonitrogenous amino acids. Intravenous [2H5]-phenylalanine and [13C]-leucine were simultaneously infused. After GC-MS analysis, whole body protein turnover was estimated from traced plasma amino acids fluxes and the fractional synthesis rate (FSR) of gut mucosal protein was calculated from protein and intracellular phenylalanine and leucine enrichments in duodenal biopsies. The mRNA levels for ubiquitin, cathepsin D and m-calpain were analysed in biopsies by RT-PCR. Glutamine significantly increased mucosal protein FSR as compared to saline. Glutamine and amino acids had similar effects on FSR. The mRNA level for ubiquitin was significantly decreased after glutamine infusion as compared both to saline and amino acids, while cathepsin D and m-calpain mRNA levels were not affected. Enteral glutamine stimulates mucosal protein synthesis and may attenuate ubiquitin-dependent proteolysis, and thus improve protein balance in human gut.




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