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Articles in PresS, published online ahead of print February 20, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00406.2001
Submitted on September 17, 2001
Accepted on January 23, 2002
1 G.I.Diseases Research Unit, Queen's University, Kingston, Ontario, Canada
* To whom correspondence should be addressed. E-mail: vanners{at}hdh.kari.net.
This study examines whether intestinal inflammation leads to changes in the properties of ion channels in dorsal root ganglia (DRG) neurons. Ileitis was induced by injection of trinitrobenzene sulfonic acid (TNBS) and DRG neurons innervating the ileum were labeled using Fast Blue. Intracellular recording techniques were used to measure electrophysiological properties of acutely dissociated neurons, 12-24 hours after dissection. Nociceptive neurons were identified by sensitivity to capsaicin, tetrodotoxin resistance, and size (<30 µm). The action potential threshold in neurons from TNBS treated animals was reduced by >70% compared to controls (p<0.001) but the resting membrane potential was unchanged. Cell diameter, input resistance (67%) and action potential upstroke velocity (22%) increased in the TNBS group (p<0.05). The number of action potentials discharged increased in the TNBS group (p<0.001), while application of 4-aminopyridine to control cells mimicked this effect. This study demonstrates that ileitis induces hyperexcitability in nociceptive DRG neurons and changes in the properties of Na+ and K+ channels at the soma, which persist after removal from the inflamed environment.
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