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Am J Physiol Gastrointest Liver Physiol (March 20, 2002). doi:10.1152/ajpgi.00420.2001
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Articles in PresS, published online ahead of print March 20, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00420.2001
Submitted on October 1, 2001
Accepted on March 14, 2002

Multiple Transcription Factors in the 5'-Flanking Region of the Human Polymeric Ig Receptor Control its Basal Expression

R. Sergio Solorzano-Vargas1, Jiafang Wang2, Lingling Jiang2, Hugh V Tsai2, Luis O Ontiveros2, Mukta A Vazir1, Renato J Aguilera3, and Martin G Martin2*

1 Department of Biology, California State University Northridge, Northridge, CA, USA; Departments of Pediatrics, Division of Gastroenterology and Nutrition, University of California Los Angeles School of Medicine, Los Angeles, CA, USA
2 Departments of Pediatrics, Division of Gastroenterology and Nutrition, University of California Los Angeles School of Medicine, Los Angeles, CA, USA
3 Department of Molecular Cellular and Developmental Biology, University of California Los Angeles School of Medicine, Los Angeles, CA, USA

* To whom correspondence should be addressed. E-mail: mmartin{at}mednet.ucla.edu.

The polymeric Ig receptor (pIgR) is a critical component of the mucosal immune system and is expressed in largest amounts in the small intestine. In this study, we describe the initial characterization of the core promoter region of this gene. Expression of chimeric promoter-reporter constructs was supported in Caco2 and HT-29 cells, and DNase I footprint analysis revealed a large protein complex within the core promoter region. Site directed mutagenesis experiments determined that elements within this region serve to either augment or repress basal activity of the human pIgR promoter. Band shift assays of overlapping oligonucleotides within the core promoter identified eight distinct complexes; the abundance of most complexes was enhanced in post-confluent cells. In summary, we report the characterization of the human pIgR promoter and the essential role that eight different nuclear complexes have in controlling basal expression of this gene in Caco2 cells.




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 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Jiang, J. Wang, R. S. Solorzano-Vargas, H. V. Tsai, E. M Gutierrez, L. O. Ontiveros, P. R. Kiela, S. V. Wu, and M. G. Martin
Characterization of the rat intestinal Fc receptor (FcRn) promoter: transcriptional regulation of FcRn gene by the Sp family of transcription factors
Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G922 - G931.
[Abstract] [Full Text] [PDF]


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