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TRANSLOCATION IN ENTERIC NEURONS AND INTERSTITIAL CELLS OF CAJAL IN RESPONSE TO MUSCARINIC STIMULATION
1 Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, USA
2 Department of Pharmacology, University of Nevada School of Medicine, Reno, NV, USA
* To whom correspondence should be addressed. E-mail: kent{at}physio.unr.edu.
Interstitial cells of Cajal in the deep muscular plexus (ICC-DMP) of the small intestine express excitatory neurotransmitter receptors. We tested whether ICC-DMP are functionally innervated by cholinergic neurons in the murine intestine. Muscles were stimulated by intrinsic nerves and acetylcholine and processed for immunohistochemistry to determine these effects on protein kinase C
(PKC) activation. Under control conditions, PKC-LI was only observed in myenteric neurons within the tunica muscularis. Electrical field stimulation or ACh caused translocation of neural PKC-LI from the cytosol to a peripheral compartment. After stimulation, PKC-LI was found in spindle-shaped cells in the deep muscular plexus. These cells were identified as ICC-DMP by Kit-LI and vimentin-LI. PKC-LI in ICC-DMP and translocation of PKC-LI in neurons was blocked by tetrodotoxin or atropine suggesting these responses was due to activation of muscarinic receptors. Western blots also confirmed translocation of PKC-LI. In conclusion, PKC translocation is linked to muscarinic receptor activation in ICC-DMP and a sub-population of myenteric neurons. These studies demonstrate that ICC-DMP are functionally innervated by excitatory motor neurons.
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