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Articles in PresS, published online ahead of print January 9, 2002
Am J Physiol Gastrointest Liver Physiol, 10.1152/ajpgi.00434.2001
Submitted on October 10, 2001
Accepted on January 7, 2002
1 Medicine, Digestive Diseases, UCLA, Los Angeles, CA, USA
* To whom correspondence should be addressed. E-mail: ytache{at}ucla.edu.
Peripheral CRF activates myenteric neurons in the proximal colon through CRF1 receptor in conscious rats. AM J Physiol: Gastrointest Liver Physiol.-Corticotropin-releasing factor (CRF) injected peripherally induces clustered spike burst activity in the proximal colon through CRF1 receptors in rats. We investigated the effect of intraperitoneal (ip) CRF on proximal colon ganglionic myenteric cell activity in conscious rats using Fos immunohistochemistry on the colonic longitudinal muscle/myenteric plexus whole mount preparation. In vehicle pretreated rats, there were only a few Fos immunoreactive (IR) cells/ganglion (1.2 ± 0.6). CRF (10 µg/kg, ip) induced Fos expression in 19.6 ± 2.1 cells/ganglion. The CRF1/CRF2 antagonist, astressin (33 µg/kg, ip) and the selective CRF1 antagonist CP-154,526 (20 mg/kg, subcutaneously, sc) prevented ip CRF-induced Fos expression in the proximal colon (number of Fos-IR cells/ganglion: 2.7 ± 1.2 and 1.0 ± 1.0 respectively) while atropine (1 mg/kg, sc) had no effect. Double labeling of Fos with protein gene product 9.5 revealed the neuronal identity of activated cells that were all encircled by varicose fibers-IR to vesicular acetylcholine transporter. Fos immunoreactivity was mainly present in ChAT-IR nerve cell bodies but not in the NADPH-diaphorase positive cells. These results indicate that peripheral CRF activates myenteric cholinergic neurons in the proximal colon through CRF1 receptor.
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