| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Gastroenterology, Hepatology & General Medicine, Royal Adelaide Hospital, Nerve-Gut Research Laboratory, Hanson Institute, Adelaide, South Australia, Australia; Discipline of Physiology, School of Molecular & Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia
2 Department of Gastroenterology, Hepatology & General Medicine, Royal Adelaide Hospital, Nerve-Gut Research Laboratory, Hanson Institute, Adelaide, South Australia, Australia; Discipline of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, South Australia, Australia
3 Department of Gastroenterology, Hepatology & General Medicine, Royal Adelaide Hospital, Nerve-Gut Research Laboratory, Hanson Institute, Adelaide, South Australia, Australia
4 Discipline of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, South Australia, Australia
5 Department of Gastroenterology, Hepatology & General Medicine, Royal Adelaide Hospital, Nerve-Gut Research Laboratory, Hanson Institute, Adelaide, South Australia, Australia; Discipline of Physiology, School of Molecular & Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia; Discipline of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, South Australia, Australia
* To whom correspondence should be addressed. E-mail: richard.young{at}adelaide.edu.au.
Nutrient-evoked gastrointestinal reflexes are likely initiated by specialized epithelial cells located in the small intestine that detect luminal stimuli and release mediators that activate vagal endings. The G-protein, 
-gustducin, a key signal molecule in lingual taste detection, has been identified in mouse small intestine where it may also subserve nutrient detection; however the phenotype of -gustducin cells is unknown. Immunohistochemistry was performed throughout the mouse small intestine for -gustducin, enteroendocrine cell markers 5-HT and glucagon-like peptide-1 (GLP 1), and brush cell markers neuronal nitric oxide synthase (nNOS) and UEA-1 lectin binding, singly, and in combination. -gustducin was expressed in solitary epithelial cells of the mid-upper villus, which were distributed in a regional manner with most occurring within the mid-jejunum. Here, 27% of -gustducin cells co-labeled for 5-HT and 15% for GLP-1; 57% of -gustducin cells co-labeled UEA-1, with no triple labeling. -gustducin cells that co-labeled for 5-HT or GLP-1 were of distinct morphology and exhibited a different -gustducin immunolabeling pattern to those co-labeled with UEA-1. nNOS was absent from intestinal epithelium despite strong labeling in the myenteric plexus. We conclude that subsets of enteroendocrine cells in the mid-jejunum and brush cells (more generally distributed) are equipped to utilise -gustducin signaling in mice. Intestinal taste modalities may be signaled by these enteroendocrine cells via the release of 5 HT, GLP-1 or co-expressed mediators, or by brush cells via a non-nitrergic mediator in distinct regions of the intestine.
This article has been cited by other articles:
|
|
 |
|
  T. J. Little, N. Gupta, R. M. Case, D. G. Thompson, and J. T. McLaughlin Sweetness and bitterness taste of meals per se does not mediate gastric emptying in humans Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2009; 297(3): R632 - R639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kaji, S.-i. Karaki, Y. Fukami, M. Terasaki, and A. Kuwahara Secretory effects of a luminal bitter tastant and expressions of bitter taste receptors, T2Rs, in the human and rat large intestine Am J Physiol Gastrointest Liver Physiol, May 1, 2009; 296(5): G971 - G981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ma, M. Bellon, J. M. Wishart, R. Young, L. A. Blackshaw, K. L. Jones, M. Horowitz, and C. K. Rayner Effect of the artificial sweetener, sucralose, on gastric emptying and incretin hormone release in healthy subjects Am J Physiol Gastrointest Liver Physiol, April 1, 2009; 296(4): G735 - G739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R L Young, K Sutherland, N Pezos, S M Brierley, M Horowitz, C K Rayner, and L A Blackshaw Expression of taste molecules in the upper gastrointestinal tract in humans with and without type 2 diabetes Gut, March 1, 2009; 58(3): 337 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hao, M. Dulake, E. Espero, C. Sternini, H. E. Raybould, and L. Rinaman Central Fos expression and conditioned flavor avoidance in rats following intragastric administration of bitter taste receptor ligands Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R528 - R536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Fujita, R. D. Wideman, M. Speck, A. Asadi, D. S. King, T. D. Webber, M. Haneda, and T. J. Kieffer Incretin release from gut is acutely enhanced by sugar but not by sweeteners in vivo Am J Physiol Endocrinol Metab, March 1, 2009; 296(3): E473 - E479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kidd, I. M. Modlin, B. I. Gustafsson, I. Drozdov, O. Hauso, and R. Pfragner Luminal regulation of normal and neoplastic human EC cell serotonin release is mediated by bile salts, amines, tastants, and olfactants Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G260 - G272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hao, C. Sternini, and H. E. Raybould Role of CCK1 and Y2 receptors in activation of hindbrain neurons induced by intragastric administration of bitter taste receptor ligands Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R33 - R38. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
