|
|
||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 VA GLHS / Digestive Diseases Division, CURE Center for Digestive Disease Research / UCLA School of Medicine, Los Angeles, CA, USA
2 Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
3 Division of Gastroenterology, Harbor UCLA Medical Center, Torrance, CA, USA
* To whom correspondence should be addressed. E-mail: jreeve{at}ucla.edu.
CCK-58 differs from CCK-8 in patterns of expression of pancreatic secretion of fluid and amylase, and gallbladder contraction. These differences have physiological relevance only if CCK-58 release is stimulated by nutrients entering the intestine and if CCK-58 circulates in sizeable amounts. In this study, we report that when radiolabeled CCK-58 is added to rat blood and plasma is formed, there is extensive loss and degradation of the radioactive peptide. Therefore, a new method was designed to minimize loss and degradation of this label. This method recovered more than 85% of the label with no detectable degradation. Furthermore, the optimized method recovered all exogenous cholecystokinin molecular forms in greater than 80% yields. Blood from fasted rats and rats where cholecystokinin release was stimulated by trypsin inhibitor, camostat contained only CCK-58 (3.5 ± 0.5 fmol/mL and 17 ± 1.5 fmol/mL, respectively). Because CCK-58 predominates in the blood this molecular form should be used in studies on the physiology and pathophysiology of cholecystokinin.
This article has been cited by other articles:
![]() |
S. V. Wu, K. G. Harikumar, R. J. Burgess, J. R. Reeve Jr., and L. J. Miller Effects of cholecystokinin-58 on type 1 cholecystokinin receptor function and regulation Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G641 - G647. [Abstract] [Full Text] [PDF] |
||||
![]() |
C.-M. Lo, L. C. Samuelson, J. B. Chambers, A. King, J. Heiman, R. J. Jandacek, R. R. Sakai, S. C. Benoit, H. E. Raybould, S. C. Woods, et al. Characterization of mice lacking the gene for cholecystokinin Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R803 - R810. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. W. Adelson, H. P. Kosoyan, Y. Wang, J. Z. Steinberg, and Y. Tache Gastric Vagal Efferent Inhibition Evoked by Intravenous CRF Is Unrelated to Simultaneously Recorded Vagal Afferent Activity in Urethane-Anesthetized Rats J Neurophysiol, April 1, 2007; 97(4): 3004 - 3014. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Yamamoto, J. R. Reeve Jr., and G. M. Green Supramaximal CCK-58 does not induce pancreatitis in the rat: role of pancreatic water secretion Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G964 - G974. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. N. Sullivan, S. J. Raboin, S. Gulley, N. T. Sinzobahamvya, G. M. Green, J. R. Reeve Jr., and A. I. Sayegh Endogenous cholecystokinin reduces food intake and increases Fos-like immunoreactivity in the dorsal vagal complex but not in the myenteric plexus by CCK1 receptor in the adult rat Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1071 - R1080. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. J. Wang and Z. J. Cui How does cholecystokinin stimulate exocrine pancreatic secretion? From birds, rodents, to humans Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R666 - R678. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Eisen, R. J. Phillips, N. Geary, E. A. Baronowsky, T. L. Powley, and G. P. Smith Inhibitory effects on intake of cholecystokinin-8 and cholecystokinin-33 in rats with hepatic proper or common hepatic branch vagal innervation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R456 - R462. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Yamamoto, J. R. Reeve Jr., D. A. Keire, and G. M. Green Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8 Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G866 - G879. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. R. Reeve Jr., R. A. Liddle, D. C. McVey, S. R. Vigna, T. E. Solomon, D. A. Keire, G. Rosenquist, J. E. Shively, T. D. Lee, P. Chew, et al. Identification of nonsulfated cholecystokinin-58 in canine intestinal extracts and its biological properties Am J Physiol Gastrointest Liver Physiol, August 1, 2004; 287(2): G326 - G333. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. R. Reeve Jr., S. V. Wu, D. A. Keire, K. Faull, P. Chew, T. E. Solomon, G. M. Green, and T. Coskun Differential bile-pancreatic secretory effects of CCK-58 and CCK-8 Am J Physiol Gastrointest Liver Physiol, March 1, 2004; 286(3): G395 - G402. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |