AJP - GI AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G501-G507, 2002. First published December 19, 2001; doi:10.1152/ajpgi.00388.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/G501    most recent
00388.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, Z.
Right arrow Articles by Gorelick, F. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, Z.
Right arrow Articles by Gorelick, F. S.
Vol. 282, Issue 3, G501-G507, March 2002

Alcohols enhance caerulein-induced zymogen activation in pancreatic acinar cells

Zhao Lu, Suresh Karne, Thomas Kolodecik, and Fred S. Gorelick

Departments of Internal Medicine, Section of Digestive Diseases, and Cell Biology, Veterans Administration Connecticut Healthcare, West Haven and Yale University School of Medicine, New Haven, Connecticut 06516

Activation of zymogens within the pancreatic acinar cell is an early feature of acute pancreatitis. Supraphysiological concentrations of cholecystokinin (CCK) cause zymogen activation and pancreatitis. The effects of the CCK analog, caerulein, and alcohol on trypsin and chymotrypsin activation in isolated pancreatic acini were examined. Caerulein increased markers of zymogen activation in a time- and concentration-dependent manner. Notably, trypsin activity reached a peak value within 30 min, then diminished with time, whereas chymotrypsin activity increased with time. Ethanol (35 mM) sensitized the acinar cells to the effects of caerulein (10-10 to 10-7 M) on zymogen activation but had no effect alone. The effects of ethanol were concentration dependent. Alcohols with a chain length of >= 2 also sensitized the acinar cell to caerulein; the most potent was butanol. Branched alcohols (2-propanol and 2-butanol) were less potent than aliphatic alcohols (1-propanol and 1-butanol). The structure of an alcohol is related to its ability to sensitize acinar cells to the effects of caerulein on zymogen activation.

trypsinogen activation peptide; trypsin; chymotrypsin; ethanol; pancreatitis


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. U. Shah, A. Sarwar, A. I. Orabi, S. Gautam, W. M. Grant, A. J. Park, A. U. Shah, J. Liu, P. K. Mistry, D. Jain, et al.
Protease activation during in vivo pancreatitis is dependent on calcineurin activation
Am J Physiol Gastrointest Liver Physiol, November 1, 2009; 297(5): G967 - G973.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Z. Husain, W. M. Grant, F. S. Gorelick, M. H. Nathanson, and A. U. Shah
Caerulein-induced intracellular pancreatic zymogen activation is dependent on calcineurin
Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1594 - G1599.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Satoh, A. S. Gukovskaya, J. R. Reeve Jr, T. Shimosegawa, and S. J. Pandol
Ethanol sensitizes NF-{kappa}B activation in pancreatic acinar cells through effects on protein kinase C-{epsilon}
Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G432 - G438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. C. Thrower, A. P. E. Diaz de Villalvilla, T. R. Kolodecik, and F. S. Gorelick
Zymogen activation in a reconstituted pancreatic acinar cell system
Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G894 - G902.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
G Perides, X Tao, N West, A Sharma, and M L Steer
A mouse model of ethanol dependent pancreatic fibrosis
Gut, October 1, 2005; 54(10): 1461 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Z. Lu, T. R. Kolodecik, S. Karne, M. Nyce, and F. Gorelick
Effect of ligands that increase cAMP on caerulein-induced zymogen activation in pancreatic acini
Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G822 - G828.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online