AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 282: G757-G765, 2002. First published February 20, 2002; doi:10.1152/ajpgi.00152.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/G757    most recent
00152.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yue, G.
Right arrow Articles by Eaton, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yue, G.
Right arrow Articles by Eaton, D. C.
Vol. 282, Issue 5, G757-G765, May 2002

Cryptdin 3 forms anion selective channels in cytoplasmic membranes of human embryonic kidney cells

Gang Yue1, Didier Merlin2, Michael E. Selsted3, Wayne I. Lencer4, James L. Madara2, and Douglas C. Eaton1

1 Center for Cell and Molecular Signaling and 2 Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322; 3 Department of Pathology, University of California Irvine, Irvine, California 92697; and 4 Department of Pediatrics, Harvard Medical School and Children's Hospital, Boston, Massachusetts 02115

Cryptdins are antimicrobial peptides secreted by Paneth cells located at the base of intestinal crypts. In addition to their antimicrobial function, cryptdins may also regulate salt and water secretion by intestinal epithelial cells. Recent work with short-circuit current measurements indicated that at least one cryptdin peptide, cryptdin 3, induces apical conductance(s) in Cl- secretory, including cystic fibrosis, epithelia. In the present study, we characterized the cryptdin 3-induced anion channel activity in human embryonic kidney (HEK) cells with single-channel patch-clamp techniques. The patch pipette was filled with solution containing different concentrations of cryptdin 3, and, after gigaseal formation, the channel activity was recorded with either cell-attached or inside-out patch modes. We found an anion selective channel with a conductance of 15 pS and open probability of 0.19, regardless of cryptdin 3 concentration. The mean open and closed times varied with the cryptdin 3 concentration. For cryptdin 3 concentrations of 10, 4, 1, and 0.5 µg/ml in the pipette, the corresponding mean open times were 1.2, 7.0, 9.0, and 17.4 ms and the corresponding mean closed times were 1.1, 1.6, 4.2, and 12.5 ms. These results suggest that cryptdin 3 forms anion-selective channels on the cytoplasmic membrane of HEK cells and that the kinetics of one such channel are affected by its interaction with other such channels.

patch clamp; chloride channels; defensins


This article has been cited by other articles:


Home page
 J. Virol.Home page
F. Groot, R. W. Sanders, O. ter Brake, K. Nazmi, E. C. I. Veerman, J. G. M. Bolscher, and B. Berkhout
Histatin 5-derived Peptide with improved fungicidal properties enhances human immunodeficiency virus type 1 replication by promoting viral entry.
J. Virol., September 1, 2006; 80(18): 9236 - 9243.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. L. Bevins
Events at the Host-Microbial Interface of the Gastrointestinal Tract V. Paneth cell {alpha}-defensins in intestinal host defense
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G173 - G176.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Tanabe, A. J. Ouellette, M. J. Cocco, and W. E. Robinson Jr.
Differential Effects on Human Immunodeficiency Virus Type 1 Replication by {alpha}-Defensins with Comparable Bactericidal Activities
J. Virol., November 1, 2004; 78(21): 11622 - 11631.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Hsia and G. A. Cornwall
DNA Microarray Analysis of Region-Specific Gene Expression in the Mouse Epididymis
Biol Reprod, February 1, 2004; 70(2): 448 - 457.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online