AJP - GI AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 283: G222-G229, 2002. First published March 13, 2002; doi:10.1152/ajpgi.00534.2001
0193-1857/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/G222    most recent
00534.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knütter, I.
Right arrow Articles by Brandsch, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knütter, I.
Right arrow Articles by Brandsch, M.
Vol. 283, Issue 1, G222-G229, July 2002

H+-peptide cotransport in the human bile duct epithelium cell line SK-ChA-1

Ilka Knütter1,2, Isabel Rubio-Aliaga3, Michael Boll3, Gerd Hause2, Hannelore Daniel3, Klaus Neubert1, and Matthias Brandsch2

1 Institute of Biochemistry, Department of Biochemistry/Biotechnology and 2 Biozentrum of the Martin Luther University Halle-Wittenberg, Halle D-06120; and 3 Molecular Nutrition Unit, Institute of Nutritional Sciences, Technical University of Munich, Freising-Weihenstephan, D-85350 Germany

This study describes for the first time the presence of H+-peptide cotransport in cells of the bile duct. Uptake of [glycine-1-14C]glycylsarcosine ([14C]Gly-Sar) in human extrahepatic cholangiocarcinoma SK-ChA-1 cells was stimulated sevenfold by an inwardly directed H+ gradient. Transport was mediated by a low-affinity system with a transport constant (Kt) value of 1.1 mM. Several dipeptides, cefadroxil, and delta -aminolevulinic acid, but not glycine and glutathione, were strong inhibitors of Gly-Sar uptake. SK-ChA-1 cells formed tight, polarized monolayers on permeable membranes. The transepithelial electrical resistance was 856 ± 29 Omega  × cm2. The transepithelial flux of [14C]Gly-Sar in apical-to-basolateral direction exceeded the basolateral-to-apical flux 11-fold. Uptake was 20-fold higher from the apical side. RT-PCR analysis using primer pairs specific for the intestinal-type peptide transporter (PEPT1) or kidney-type (PEPT2) revealed that the transport system expressed in SK-ChA-1 and also in cells of the native rabbit bile duct is PEPT1. Immunohistochemistry localized PEPT1 to the apical membrane of cholangiocytes of mouse extrahepatic biliary duct. We conclude that the cells of the mammalian extrahepatic biliary tract epithelium express the intestinal-type H+-peptide cotransporter in their apical membrane. SK-ChA-1 cells represent a convenient model to study the physiological and clinical aspects of peptide transport in cholangiocytes.

membrane transport; peptide symporter; peptide transporter-1; cell culture


This article has been cited by other articles:


Home page
 J ANIM SCIHome page
E. R. Gilbert, E. A. Wong, and K. E. Webb Jr.
BOARD-INVITED REVIEW: Peptide absorption and utilization: Implications for animal nutrition and health
J Anim Sci, September 1, 2008; 86(9): 2135 - 2155.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
K. Mitsuoka, S. Miyoshi, Y. Kato, Y. Murakami, R. Utsumi, Y. Kubo, A. Noda, Y. Nakamura, S. Nishimura, and A. Tsuji
Cancer Detection Using a PET Tracer, 11C-Glycylsarcosine, Targeted to H+/Peptide Transporter
J. Nucl. Med., April 1, 2008; 49(4): 615 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. T. T. Nguyen, L. Charrier-Hisamuddin, G. Dalmasso, A. Hiol, S. Sitaraman, and D. Merlin
Association of PepT1 with lipid rafts differently modulates its transport activity in polarized and nonpolarized cells
Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1155 - G1165.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. A. Groneberg, A. Fischer, K. F. Chung, and H. Daniel
Molecular Mechanisms of Pulmonary Peptidomimetic Drug and Peptide Transport
Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 251 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Daniel and I. Rubio-Aliaga
An update on renal peptide transporters
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F885 - F892.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. Neumann and M. Brandsch
delta -Aminolevulinic Acid Transport in Cancer Cells of the Human Extrahepatic Biliary Duct
J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 219 - 224.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online